PUBLICATION
Generation of rainbow color transgenic zebrafish
- Authors
- Gong, Z.
- ID
- ZDB-PUB-030103-12
- Date
- 2001
- Source
- 6th Asian Fisheries Forum Book of Abstracts : p. 85 (Abstract)
- Registered Authors
- Gong, Zhiyuan
- Keywords
- Gene transfer; Color; Green fluorescent protein; red fluorescent protein; transgenic fish; yellow fluorescent protein; Colour; Ornamental fish; Tropical fish; Aquarium culture; Bioluminescence; Genes; Biotechnology; Fluorescence; Danio rerio
- MeSH Terms
- none
- PubMed
- none
Citation
Gong, Z. (2001) Generation of rainbow color transgenic zebrafish. 6th Asian Fisheries Forum Book of Abstracts. :p. 85.
Abstract
The transgenic technology has been widely used in biotechnology, from generation of genetically modified (GM) foods to production of pharmaceutical proteins. Inspired by the success of generation of supermice using exogenously growth hormone gene, the transgenic technology has been used successfully in generation of fast growing fish by transferring a growth hormone gene. However, because of the current controversies over GM foods, the marketing of such growth hormone transgenic fish remains to face an uphill battle. In the present study, we attempted to use the transgenic technology to develop novel varieties of ornamental fish by transferring a jellyfish green fluorescent protein (gfp) gene. We used the zebrafish (Danio rerio) as a research model. The initial work was to use a gfp gene under a skin-specific promoter from a cytokeratin gene, krt8, and a promoter from a muscle-specific gene, mylz2, encoding a myosin light chain 2 polypeptide. Stable lines of gfp transgenic zebrafish were obtained from both gene constructs and the expression patterns of GFP mimicked the endogenous expression of the two genes. As the expression of GFP under the krt8 promoter was only limited to a single layer of epidermis, the green fluorescent color was observed only under a fluorescent microscope.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping