ZFIN ID: ZDB-PUB-021204-1
Reptin and pontin antagonistically regulate heart growth in zebrafish embryos
Rottbauer, W., Saurin, A.J., Lickert, H., Shen, X., Burns, C.G., Wo, Z.G., Kemler, R., Kingston, R., Wu, C., and Fishman, M.
Date: 2002
Source: Cell   111(5): 661-672 (Journal)
Registered Authors: Fishman, Mark C., Rottbauer, Wolfgang
Keywords: none
MeSH Terms:
  • ATP-Dependent Proteases
  • Adenosine Triphosphatases/chemistry
  • Adenosine Triphosphatases/physiology
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cardiomegaly/embryology
  • Cardiomegaly/etiology
  • Cardiomegaly/genetics
  • Carrier Proteins
  • Cell Division
  • Conserved Sequence
  • Cytoskeletal Proteins/physiology
  • Embryo, Nonmammalian
  • Enzyme Activation
  • Gene Expression Regulation, Developmental
  • Heart/embryology*
  • Heat-Shock Proteins/physiology
  • Molecular Sequence Data
  • Myocardium/cytology
  • Myocardium/ultrastructure
  • Nuclear Proteins/genetics
  • Nuclear Proteins/physiology*
  • Point Mutation
  • Sequence Homology, Amino Acid
  • Serine Endopeptidases/physiology
  • Trans-Activators/physiology
  • Zebrafish
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • beta Catenin
PubMed: 12464178 Full text @ Cell
Organ size is precisely regulated during development, but the control mechanisms remain obscure. We have isolated a mutation in zebrafish, liebeskummer (lik), which causes development of hyperplastic embryonic hearts. lik encodes Reptin, a component of a DNA-stimulated ATPase complex. The mutation activates ATPase activity of Reptin complexes and causes a cell-autonomous proliferation of cardiomyocytes to begin well after progenitors have fashioned the primitive heart tube. With regard to heart growth, beta-catenin and Pontin, a DNA-stimulated ATPase that is often part of complexes with Reptin, are in the same genetic pathways. Pontin reduction phenocopies the cardiac hyperplasia of the lik mutation. Thus, the Reptin/Pontin ratio serves to regulate heart growth during development, at least in part via the beta-catenin pathway.