ZFIN ID: ZDB-PUB-021112-4
A defect in a novel Nek-family kinase causes cystic kidney disease in the mouse and in zebrafish
Liu, S., Lu, W., Obara, T., Kuida, S., Lehoczky, J., Dewar, K., Drummond, I.A., and Beier, D.R.
Date: 2002
Source: Development (Cambridge, England)   129(24): 5839-5846 (Journal)
Registered Authors: Beier, David R., Drummond, Iain, Obara, Tomoko
Keywords: PKD, mouse models, zebrafish, Nek kinase
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • COS Cells
  • Cell Nucleus/metabolism
  • Cloning, Molecular
  • Databases as Topic
  • Disease Models, Animal
  • Genome
  • Kidney Diseases, Cystic/genetics*
  • Mice
  • Mice, Inbred C57BL
  • Models, Genetic
  • Molecular Sequence Data
  • Mutation
  • Oligonucleotides, Antisense/pharmacology
  • Physical Chromosome Mapping
  • Protein Kinases*
  • Protein-Serine-Threonine Kinases/genetics*
  • Protein-Serine-Threonine Kinases/physiology*
  • RNA, Messenger/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time Factors
  • Zebrafish
  • Zebrafish Proteins
PubMed: 12421721 Full text @ Development
The murine autosomal recessive juvenile cystic kidney (jck) mutation results in polycystic kidney disease. We have identified in jck mice a mutation in Nek8, a novel and highly conserved member of the Nek kinase family. In vitro expression of mutated Nek8 results in enlarged, multinucleated cells with an abnormal actin cytoskeleton. To confirm that a defect in the Nek8 gene can cause cystic disease, we performed a cross-species analysis: injection of zebrafish embryos with a morpholino anti-sense oligonucleotide corresponding to the ortholog of Nek8 resulted in the formation of pronephric cysts. These results demonstrate that comparative analysis of gene function in different model systems represents a powerful means to annotate gene function.