Molecular Embryology: Methods and Protocols

Authors

Sharpe, P.T. and Mason, I.

ID

ZDB-PUB-021016-126

Date

1999

Source

Totowa, NJ: Humana Press : 756p (Book) Generate reference

Registered Authors

Mason, Ivor

Keywords

Pt.1. The mouse embryo -- The mouse as a developmental model -- Culture of postimplantation mouse embryos -- Organ culture in the analysis of tissue interactions -- Treatment of mice with retinoids in vivo and in vitro: skeletal staining -- Cell grafting and labeling in postimplantation mouse embryos -- Production of transgenic rodents by the microinjection of coloned DNA into fertilized one-celled eggs -- Cre recombinase mediated alterations of the mouse genome using embryonic stem cells -- Gene trapping in mouse embryonic stem cells -- Production and growth of conditionally immortal cell lines from the H-2KbtsA58 transgenic mouse -- Reporter genes for the study of transcriptional regulation in transgenic mouse embryos -- Application of IacZ transgenic mice to cell lineage studies -- Mouse primordial germ cells: isolation and in vitro cultue -- Pt.2. The chicken embryo -- The avian embryo: an overview -- Chick embryos: incubation and isolation -- New culture -- Grafting Hensen's node -- Grafting of Somites -- Notochord grafts -- Transplantation of avian neural tissue -- Grafting of apical ridge and polarizing region --- Tissue recombinations in collagen gels -- Quail-chick chimeras -- Using fluorescent dyes for fate mapping, lineage analysis, and axon tracing in the chick embryo -- Pt.3. Amphibian embryo -- An overview of Xenopus development -- Mesoderm induction assays -- Experimental embryological methods for analysis of neural induction in the amphibian -- A method for generating transgenic frog embryos -- Axolotl/newt -- Pt.4. Zebrafish -- Pt.5. Nonvertebrate chordates -- Pt.6. Retroviruses -- Pt.7. Molecular techniques -- Pt.8. Microscopy and photography.

MeSH Terms

none

PubMed

none