PUBLICATION
Expressed sequence tag analysis of expression profiles of zebrafish testis and ovary
- Authors
- Zeng, S. and Gong, Z.
- ID
- ZDB-PUB-021002-1
- Date
- 2002
- Source
- Gene 294(1-2): 45-53 (Journal)
- Registered Authors
- Gong, Zhiyuan
- Keywords
- dynein intermediate chain 3; germ cell; gonad; oocyte; spermatocyte; zona pellucida protein
- MeSH Terms
-
- Animals
- Blotting, Northern
- DNA, Complementary/chemistry
- DNA, Complementary/genetics
- Expressed Sequence Tags*
- Female
- Gene Expression Profiling*
- Gene Library
- Male
- Molecular Sequence Data
- Ovary/metabolism*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Sequence Analysis, DNA
- Testis/metabolism*
- Zebrafish/genetics*
- PubMed
- 12234666 Full text @ Gene
Citation
Zeng, S. and Gong, Z. (2002) Expressed sequence tag analysis of expression profiles of zebrafish testis and ovary. Gene. 294(1-2):45-53.
Abstract
In the present study, two gonad cDNA libraries from zebrafish testes and ovaries were constructed and a total of 1025 expressed sequence tag ( EST) clones were generated from the two libraries: 501 from the testis library and 524 from the ovary library. A total of 641 of the EST clones were identified to share significant sequence identity with known sequences in GenBank, representing at least 478 different zebrafish genes. In order to understand the molecular compositions of the two gonad organs, the expression profiles of the identified clones in these two gonad cDNA libraries were analyzed. Both gonad libraries have a higher portion of clones for nuclear proteins and a lower portion for proteins in translational machinery, cytoskeleton and mitochondria than our previously characterized whole-adult cDNA library. Most abundant cDNA clones in the two gonad libraries were identified and over 10% of ovary clones were found to encode egg membrane proteins (zona pellucida or ZP proteins). Furthermore, the testis library showed a more even distribution of cDNA clones with relatively fewer abundant clones that tend to contribute redundant clones in EST projects; thus, the testis library can supply more unique and novel cDNA sequences in a zebrafish EST project. Another aim of this study is to identify cDNA clones that can be used as molecular markers for the analysis of the gonad development in zebrafish. Eleven potential clones were selected to analyze their expression patterns by Northern blot hybridization. Most of them showed a specific or predominant expression in the expected testis or ovary tissue. At last, four of the clones were found, by section in situ hybridization, to be expressed specifically in the germ cells of the testis or ovary and thus they are suitable molecular markers for analyses of spermatogenesis and oogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping