ZFIN ID: ZDB-PUB-020912-2
BrdU-, neuroD (nrd)- and Hu-studies reveal unusual non-ventricular neurogenesis in the postembryonic zebrafish forebrain
Mueller, T., and Wullimann, M.
Date: 2002
Source: Mechanisms of Development   117(1-2): 123-135 (Journal)
Registered Authors: Mueller, Thomas, Wullimann, Mario F.
Keywords: zebrafish; Hu-protein; 5-bromo-2'-deoxyuridine; proliferating cell nuclear antigen; neuroD; nrd; forebrain; diencephalon; preglomerular area; pretectum; posterior tuberculum; telencephalon; cerebellum; external granular layer; basic helix-loop-helix transcription; factor; mitosis; proliferation
MeSH Terms:
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • Bromodeoxyuridine/metabolism*
  • Cell Differentiation
  • Cell Division
  • ELAV Proteins
  • Immunohistochemistry
  • In Situ Hybridization
  • Larva/cytology
  • Larva/growth & development
  • Larva/metabolism
  • Mitosis
  • Nerve Tissue Proteins/genetics*
  • Neurons/cytology
  • Neurons/metabolism
  • Proliferating Cell Nuclear Antigen/metabolism
  • Prosencephalon/cytology
  • Prosencephalon/growth & development*
  • Prosencephalon/metabolism*
  • RNA-Binding Proteins/metabolism*
  • Stem Cells/cytology
  • Stem Cells/metabolism
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Zebrafish/metabolism*
PubMed: 12204253 Full text @ Mech. Dev.
In the postembryonic zebrafish forebrain, subpial locations of neurogenesis do exist in the early cerebellar external granular layer, and - unusually among vertebrates - in the primordial pretectal (M1) and preglomerular (M2) Anlagen as shown here with 5-bromo-2'-deoxyuridine ( BrdU)/Hu-immunocytochemistry and in situ hybridization of neuroD. An intermediate BrdU incubation time of 12-16h reveals in addition to proliferative ventricularly located cells those in M1 and M2. This BrdU saturation-labeling shows - in conjunction with a Hu-assay demonstrating earliest neuronal differentiation - that proliferating cells in M1 and M2 represent neuronal progenitors. This is demonstrated by single BrdU- labeled and double BrdU-/Hu-labeled cells in these aggregates. Further, expression of NeuroD - a marker for freshly determined neuronal cells - confirms this unusual subpial postembryonic forebrain neurogenesis.