|ZFIN ID: ZDB-PUB-020912-2|
BrdU-, neuroD (nrd)- and Hu-studies reveal unusual non-ventricular neurogenesis in the postembryonic zebrafish forebrain
Mueller, T., and Wullimann, M.
|Source:||Mechanisms of Development 117(1-2): 123-135 (Journal)|
|Registered Authors:||Mueller, Thomas, Wullimann, Mario F.|
|Keywords:||zebrafish; Hu-protein; 5-bromo-2'-deoxyuridine; proliferating cell nuclear antigen; neuroD; nrd; forebrain; diencephalon; preglomerular area; pretectum; posterior tuberculum; telencephalon; cerebellum; external granular layer; basic helix-loop-helix transcription; factor; mitosis; proliferation|
|PubMed:||12204253 Full text @ Mech. Dev.|
Mueller, T., and Wullimann, M. (2002) BrdU-, neuroD (nrd)- and Hu-studies reveal unusual non-ventricular neurogenesis in the postembryonic zebrafish forebrain. Mechanisms of Development. 117(1-2):123-135.
ABSTRACTIn the postembryonic zebrafish forebrain, subpial locations of neurogenesis do exist in the early cerebellar external granular layer, and - unusually among vertebrates - in the primordial pretectal (M1) and preglomerular (M2) Anlagen as shown here with 5-bromo-2'-deoxyuridine ( BrdU)/Hu-immunocytochemistry and in situ hybridization of neuroD. An intermediate BrdU incubation time of 12-16h reveals in addition to proliferative ventricularly located cells those in M1 and M2. This BrdU saturation-labeling shows - in conjunction with a Hu-assay demonstrating earliest neuronal differentiation - that proliferating cells in M1 and M2 represent neuronal progenitors. This is demonstrated by single BrdU- labeled and double BrdU-/Hu-labeled cells in these aggregates. Further, expression of NeuroD - a marker for freshly determined neuronal cells - confirms this unusual subpial postembryonic forebrain neurogenesis.