ZFIN ID: ZDB-PUB-020912-17
Furrow-specific endocytosis during cytokinesis of zebrafish blastomeres
Feng, B., Schwarz, H., and Jesuthasan, S.
Date: 2002
Source: Experimental cell research   279(1): 14-20 (Journal)
Registered Authors: Feng, Bo, Jesuthasan, Suresh
Keywords: cytokinesis; endocytosis; clathrin; caveolin; Danio rerio
MeSH Terms:
  • Animals
  • Blastomeres/chemistry
  • Blastomeres/cytology*
  • Blastomeres/ultrastructure
  • Caveolin 1
  • Caveolins/analysis
  • Cell Division/drug effects
  • Chlorpromazine/pharmacology
  • Clathrin/analysis
  • Cyclodextrins/pharmacology
  • Endocytosis*/drug effects
  • Exocytosis
  • Microscopy, Fluorescence
  • Transport Vesicles/metabolism
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
  • beta-Cyclodextrins*
PubMed: 12213209 Full text @ Exp. Cell Res.
Mutations affecting endocytosis, such as those in clathrin and dynamin, unexpectedly cause defects in cytokinesis in a number of organisms. To explore the relationship between endocytosis and cytokinesis, we used the relatively large cells of the transparent zebrafish embryo. Using fluorescent markers for fluid-phase as well as plasma membrane uptake, we demonstrate that cytokinesis involves furrow-specific endocytosis. Clathrin-coated pits are visible near the furrow in ultrathin sections, while immunolabeling demonstrates that clathrin and caveolin are localized to the cleavage furrow. Hence, it is likely that both clathrin - and caveolae-mediated endocytosis occurs at the furrow during cytokinesis. Dynamin II is also localized to the furrow and may mediate furrow-specific endocytosis. Treatment of embryos with chlorpromazine or with methyl-beta-cyclodextrin, both of which inhibit endocytosis, prevents the normal completion of cytokinesis. These data suggest that furrow-specific endocytosis is an integral part of cytokinesis.