PUBLICATION
Furrow-specific endocytosis during cytokinesis of zebrafish blastomeres
- Authors
- Feng, B., Schwarz, H., and Jesuthasan, S.
- ID
- ZDB-PUB-020912-17
- Date
- 2002
- Source
- Experimental cell research 279(1): 14-20 (Journal)
- Registered Authors
- Feng, Bo, Jesuthasan, Suresh
- Keywords
- cytokinesis; endocytosis; clathrin; caveolin; Danio rerio
- MeSH Terms
-
- Animals
- Blastomeres/chemistry
- Blastomeres/cytology*
- Blastomeres/ultrastructure
- Caveolin 1
- Caveolins/analysis
- Cell Division/drug effects
- Chlorpromazine/pharmacology
- Clathrin/analysis
- Cyclodextrins/pharmacology
- Endocytosis*/drug effects
- Exocytosis
- Microscopy, Fluorescence
- Transport Vesicles/metabolism
- Zebrafish/anatomy & histology
- Zebrafish/embryology*
- beta-Cyclodextrins*
- PubMed
- 12213209 Full text @ Exp. Cell Res.
Citation
Feng, B., Schwarz, H., and Jesuthasan, S. (2002) Furrow-specific endocytosis during cytokinesis of zebrafish blastomeres. Experimental cell research. 279(1):14-20.
Abstract
Mutations affecting endocytosis, such as those in clathrin and dynamin, unexpectedly cause defects in cytokinesis in a number of organisms. To explore the relationship between endocytosis and cytokinesis, we used the relatively large cells of the transparent zebrafish embryo. Using fluorescent markers for fluid-phase as well as plasma membrane uptake, we demonstrate that cytokinesis involves furrow-specific endocytosis. Clathrin-coated pits are visible near the furrow in ultrathin sections, while immunolabeling demonstrates that clathrin and caveolin are localized to the cleavage furrow. Hence, it is likely that both clathrin - and caveolae-mediated endocytosis occurs at the furrow during cytokinesis. Dynamin II is also localized to the furrow and may mediate furrow-specific endocytosis. Treatment of embryos with chlorpromazine or with methyl-beta-cyclodextrin, both of which inhibit endocytosis, prevents the normal completion of cytokinesis. These data suggest that furrow-specific endocytosis is an integral part of cytokinesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping