PUBLICATION
Cloned zebrafish by nuclear transfer from long-term-cultured cells
- Authors
- Lee, K-Y., Huang, H., Ju, B., Yang, Z., and Lin, S.
- ID
- ZDB-PUB-020807-11
- Date
- 2002
- Source
- Nat. Biotechnol. 20(8): 795-799 (Journal)
- Registered Authors
- Huang, Haigen, Ju, Bensheng, Lee, Ki-Young, Lin, Shuo, Yang, Zhongan
- Keywords
- none
- MeSH Terms
-
- Animals
- Cells, Cultured
- Chromosomes/genetics
- Cloning, Organism/methods*
- Embryo, Nonmammalian/embryology*
- Embryonic Development
- Female
- Fibroblasts
- Male
- Nuclear Transfer Techniques*
- Ovum/cytology
- Time Factors
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/growth & development
- PubMed
- 12134167 Full text @ Nat. Biotechnol.
Citation
Lee, K-Y., Huang, H., Ju, B., Yang, Z., and Lin, S. (2002) Cloned zebrafish by nuclear transfer from long-term-cultured cells. Nat. Biotechnol.. 20(8):795-799.
Abstract
Although mammals have been cloned from genetically manipulated cultured cells, a comparable achievement has not been realized in lower vertebrates. Here we report that fertile transgenic zebrafish can be obtained by nuclear transfer using embryonic fibroblast cells from long- term cultures. The donor nuclei, modified by retroviral insertions expressing green fluorescent protein (GFP), were transplanted into manually enucleated eggs. Overall, a 2% success rate was achieved, resulting in 11 adult transgenic zebrafish expressing GFP. These nuclear transplants produced fertile, diploid offspring, and their F1/F2 progeny continued to express GFP in a pattern identical to that of the founder fish. This finding demonstrates that slowly dividing nuclei from cultured cells can be reprogrammed to support rapid embryonic development and sets up a foundation for targeted genetic manipulation in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping