ZFIN ID: ZDB-PUB-020730-8
Platelet-derived growth factor receptor alpha (pdgfr-alpha) gene in zebrafish embryonic development
Liu, L., Chong, S.W., Balasubramaniyan, N.V., Korzh, V., and Ge, R.
Date: 2002
Source: Mechanisms of Development   116(1-2): 227-230 (Journal)
Registered Authors: Balasubramaniyan, Nataraja, Chong, Shang Wei, Korzh, Vladimir, Liu, Lihui
Keywords: pdgfr-a; zebratish; neurogenesis; somitogenesis; Kupffer's vesicle; neural crest cells (NCC
MeSH Terms:
  • Animals
  • Cloning, Molecular
  • DNA, Complementary/genetics
  • Gene Expression Regulation, Developmental
  • In Situ Hybridization
  • Neural Crest/embryology
  • Neural Crest/metabolism
  • Phylogeny
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Receptor, Platelet-Derived Growth Factor alpha/genetics*
  • Somites/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics*
PubMed: 12128230 Full text @ Mech. Dev.
Here we present the cloning of a full-length zebrafish pdgfr-alpha cDNA as well as the expression of this gene during zebrafish embryogenesis. We show that zebrafish pdgfr-alpha mRNA is present at high levels in the fertilized egg as well as in all embryonic cells up to the end of gastrulation. Spatially restricted expression of the gene started after the onset of segmentation and is mainly localized in premigratory neural crest cells, the placodes, the anterior paraxial cells of somites and the adaxial cells of the tailbud. Transient expression of this gene was also detected in the early Kupffer's vesicle, a teleost-specific structure. Expression of the zebrafish pdgfr-alpha is both conserved as well as diverged comparing to that of other vertebrate species.