PUBLICATION
Role of PTPase(s) in regulating Fyn kinase at fertilization of the zebrafish egg
- Authors
- Wu, W. and Kinsey, W.H.
- ID
- ZDB-PUB-020703-1
- Date
- 2002
- Source
- Developmental Biology 247(2): 286-94 (Journal)
- Registered Authors
- Kinsey, William H., Wu, Wen-Jun
- Keywords
- fertilization; Fyn kinase; zebrafish egg; rPTPa
- MeSH Terms
-
- Animals
- Binding Sites
- Blotting, Western
- Cell Membrane/enzymology
- Enzyme Activation
- Fertilization
- Ovum/enzymology
- Ovum/growth & development
- Precipitin Tests
- Protein Binding
- Protein Tyrosine Phosphatases/physiology*
- Proto-Oncogene Proteins/chemistry
- Proto-Oncogene Proteins/metabolism*
- Proto-Oncogene Proteins c-fyn
- Receptor-Like Protein Tyrosine Phosphatases, Class 4
- Receptors, Cell Surface*
- Zebrafish/embryology*
- Zebrafish/metabolism
- Zebrafish Proteins
- PubMed
- 12086467 Full text @ Dev. Biol.
Citation
Wu, W. and Kinsey, W.H. (2002) Role of PTPase(s) in regulating Fyn kinase at fertilization of the zebrafish egg. Developmental Biology. 247(2):286-94.
Abstract
Fertilization involves the activation of Src-family protein kinases which play a role at multiple stages of the egg activation process. The objective of the present study was to determine the mechanism by which one of these kinases, the Fyn kinase, is activated in response to fertilization of the zebrafish egg. Inhibitor studies demonstrated that many aspects of egg activation, including Fyn activation, require phosphotyrosyl phosphatase activity. A phosphotyrosyl phosphatase was found to be tightly associated with Fyn kinase and this interaction was mapped to the SH2 domain of Fyn. Coimmunoprecipitation studies identified rPTPalpha as a phosphatase that is complexed with Fyn in the egg, raising the possibility that rPTPalpha is part of the regulatory mechanism responsible for activating Fyn at fertilization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping