PUBLICATION

A dominant-negative form of p63 is required for epidermal proliferation in zebrafish

Authors
Lee, H. and Kimelman, D.
ID
ZDB-PUB-020513-5
Date
2002
Source
Developmental Cell   2(5): 607-616 (Journal)
Registered Authors
Kimelman, David, Lee, Hyunsook
Keywords
none
MeSH Terms
  • Base Sequence
  • Epidermis/embryology*
  • Animals
  • Genes, p53
  • Conserved Sequence
  • Sequence Homology, Amino Acid
  • Gene Expression Regulation, Developmental
  • Oligodeoxyribonucleotides, Antisense/genetics
  • Oligodeoxyribonucleotides, Antisense/pharmacology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Transcriptional Activation
  • Zebrafish Proteins*
  • Trans-Activators/genetics*
  • Molecular Sequence Data
  • Amino Acid Sequence
  • Membrane Proteins*
  • Mice
  • Phosphoproteins/genetics*
  • Evolution, Molecular
  • Apoptosis/genetics
(all 21)
PubMed
12015968 Full text @ Dev. Cell
Abstract
Epidermal stem cells play a critical role in producing the multilayered vertebrate skin. Products of the p63 gene not only mark the epidermal stem cells, but also are absolutely required for the formation of mammalian epidermis. We find that early zebrafish embryos express a dominant-negative form of p63 (DeltaNp63), which accumulates in the nucleus just as epidermal growth begins. Using antisense morpholino oligonucleotides, we show that DeltaNp63 is needed for epidermal growth and limb development and is specifically required for the proliferation of epidermal cells by inhibiting p53 activity. While the structure of fish epidermis is very different from that of higher vertebrates, our study shows that DeltaNp63 has essential and ancient role in the development of skin.
Genes / Markers
Figures
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Expression
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
Antibodies
Orthology
Engineered Foreign Genes
No data available
Mapping
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Citation
Lee, H. and Kimelman, D. (2002) A dominant-negative form of p63 is required for epidermal proliferation in zebrafish. Developmental Cell. 2(5):607-616.