PUBLICATION
RNA interference: mechanisms and applications
- Authors
- Fjose, A., Ellingsen, S., Wargelius, A., and Seo, H.C.
- ID
- ZDB-PUB-020410-4
- Date
- 2001
- Source
- Biotechnol. Annu. Rev. 7: 31-57 (Review)
- Registered Authors
- Ellingsen, Ståle, Fjose, Anders, Seo, Hee-Chan
- Keywords
- none
- MeSH Terms
-
- Animals
- Biotechnology
- Caenorhabditis elegans/genetics
- Caenorhabditis elegans/metabolism
- Drosophila/genetics
- Drosophila/metabolism
- Gene Silencing*
- Models, Genetic
- RNA/genetics*
- RNA/metabolism*
- RNA Processing, Post-Transcriptional
- RNA, Double-Stranded/genetics
- RNA, Double-Stranded/metabolism
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 11686048 Full text @ Biotechnol. Annu. Rev.
Citation
Fjose, A., Ellingsen, S., Wargelius, A., and Seo, H.C. (2001) RNA interference: mechanisms and applications. Biotechnol. Annu. Rev.. 7:31-57.
Abstract
RNA interference (RNAi) is a phenomenon induced by double-stranded RNA (dsRNA) in which gene expression is inhibited through specific degradation of mRNA. The mechanism involves conversion of dsRNA into short RNAs that direct ribonucleases to homologous mRNA targets. This process is related to normal defence against viruses and mobilisation of transposons. Treatment with dsRNA has become an important method for analysing gene functions in invertebrate organisms. RNAi has also been demonstrated in several vertebrate species but with lower efficiency. Development of procedures for in vivo production of dsRNA may provide efficient tools for tissue- and stage-specific gene targeting.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping