ZFIN ID: ZDB-PUB-020402-2
Regulation of alternative splicing of alpha-actinin transcript by Bruno-like proteins
Suzuki, H., Jin, Y., Otani, H., Yasuda, K., and Inoue, K.
Date: 2002
Source: Genes to cells : devoted to molecular & cellular mechanisms   7(2): 133-141 (Journal)
Registered Authors: Inoue, Kunio, Suzuki, Hiroaki
Keywords: none
MeSH Terms:
  • Actinin/genetics*
  • Alternative Splicing*
  • Amino Acid Motifs/genetics
  • Animals
  • CELF1 Protein
  • Dinucleotide Repeats/genetics
  • ELAV Proteins
  • ELAV-Like Protein 3
  • Gene Expression Regulation
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • RNA-Binding Proteins/genetics*
  • RNA-Binding Proteins/metabolism
  • Zebrafish
  • Zebrafish Proteins*
PubMed: 11895477 Full text @ Genes Cells
The Bruno-like or CELF proteins, such as mammalian CUGBP1 and Etr-3, Xenopus EDEN-BP, and Drosophila Bruno (Bru), are regulators of gene expression at the post-transcriptional level, and contain three RNA-recognition motifs (RRMs). It has been shown that mammalian CUGBP1 and Etr-3 regulate alternative splicing of cardiac troponin T pre-mRNA via binding to CUG-triplet repeats. RESULTS: Using in vitro selection and UV-crosslinking experiments, we found that zebrafish Bruno-like proteins bound to repeat elements of uridine and purine (termed UREs). It is known that non-muscle (NM) and smooth muscle (SM) exons of the rat alpha-actinin gene are used in a mutually exclusive manner. Transfection experiments in mammalian cells showed that zebrafish Brul and Etr-3 induced the muscle-specific splicing of rat alpha-actinin pre-mRNA via binding to the URE at the branch point upstream of the NM exon. In contrast, zebrafish Etr-1 promoted skipping of both the NM and SM exons in a manner which was not dependent on URE-binding. CONCLUSIONS: Our results showed that Bruno-like proteins bind to UREs and regulate the alternative splicing of alpha-actinin pre-mRNA. Members of the Bruno family play multiple roles in splicing regulation.