PUBLICATION

Forward and reverse genetic approaches to the analysis of eye development in zebrafish

Authors
Malicki, J.J., Pujic, Z., Thisse, C., Thisse, B., and Wei, X.
ID
ZDB-PUB-020227-1
Date
2002
Source
Vision Research   42(4): 527-533 (Journal)
Registered Authors
Malicki, Jarema, Pujic, Zac, Thisse, Bernard, Thisse, Christine, Wei, Xiangyun
Keywords
zebrafish; mutagenesis; screen; positional cloning; antisense; knockdown
MeSH Terms
  • Animals
  • Eye/embryology*
  • Models, Animal*
  • Mutagenesis, Insertional
  • Mutagenesis, Site-Directed
  • Oligonucleotides, Antisense
  • Retinal Degeneration/genetics*
  • Sequence Analysis, DNA
  • Zebrafish/embryology*
  • Zebrafish/genetics*
PubMed
11853769 Full text @ Vision Res.
Abstract
The zebrafish has been established as a mainstream research system, largely due to the immense success of genetic screens. Over 2000 mutant alleles affecting zebrafish's early development have been isolated in two large-scale morphological screens and several smaller efforts. So far, over 50 mutant strains display retinal defects and many more have been shown to affect the retinotectal projection. More recently, mutant isolation and characterization have been successfully followed by candidate and positional cloning of underlying genes. To supplement forward genetic mutational analysis, several reverse genetic techniques have also been developed. These recent advances, combined with the genome project, have established the zebrafish as one of the leading models for studies of visual system development.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes