PUBLICATION
The heat-inducible zebrafish hsp70 gene is expressed during normal lens development under non-stress conditions
- Authors
- Blechinger, S.R., Evans, T.G., Tang, P.T., Kuwada, J.Y., Warren, J.T., and Krone, P.H.
- ID
- ZDB-PUB-020220-4
- Date
- 2002
- Source
- Mechanisms of Development 112(1-2): 213-215 (Journal)
- Registered Authors
- Evans, Tyler, Krone, Patrick H., Kuwada, John, Tang, Ping-tao, Warren, James T., Jr.
- Keywords
- zebrafish; lens development; heat shock proteins; hsp70; eGFP; molecular chaperone; eye
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Down-Regulation
- Genes, Reporter
- Green Fluorescent Proteins
- HSP70 Heat-Shock Proteins/biosynthesis*
- HSP70 Heat-Shock Proteins/genetics*
- Hot Temperature
- In Situ Hybridization
- Lens, Crystalline/embryology*
- Luminescent Proteins/metabolism
- Promoter Regions, Genetic
- RNA, Messenger/metabolism
- Time Factors
- Transgenes
- Zebrafish
- PubMed
- 11850198 Full text @ Mech. Dev.
Citation
Blechinger, S.R., Evans, T.G., Tang, P.T., Kuwada, J.Y., Warren, J.T., and Krone, P.H. (2002) The heat-inducible zebrafish hsp70 gene is expressed during normal lens development under non-stress conditions. Mechanisms of Development. 112(1-2):213-215.
Abstract
In the present study, we show that the stress-inducible hsp70 gene in zebrafish is strongly and specifically expressed during normal lens formation from 28 to 38 hours post-fertilization, and is subsequently downregulated by 2 days of age. Only weak constitutive hsp70 mRNA signal was sporadically observed in other embryonic tissues. Similarly, transgenic fish carrying a 1.5kb fragment of the hsp70 promoter linked to eGFP exhibited fluorescence only in the lens. In contrast, both the endogenous hsp70 gene and the transgene were strongly expressed throughout the embryo following heat shock at the same developmental stages.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping