PUBLICATION
Repellent guidance of regenerating optic axons by chondroitin sulfate glycosaminoglycans in zebrafish
- Authors
- Becker, C.G. and Becker, T.
- ID
- ZDB-PUB-020220-15
- Date
- 2002
- Source
- The Journal of neuroscience : the official journal of the Society for Neuroscience 22(3): 842-853 (Journal)
- Registered Authors
- Becker, Catherina G., Becker, Thomas
- Keywords
- CNS regeneration; extracellular matrix; chondroitin sulfate proteoglycans; heparan sulfate; chondroitinase; ABC; tenascin-R; retinal ganglion cell axons; neurite outgrowth
- MeSH Terms
-
- Animals
- Axons/drug effects
- Axons/metabolism*
- Cells, Cultured
- Chondroitin ABC Lyase/pharmacology
- Chondroitin Sulfates/metabolism*
- Chondroitin Sulfates/pharmacology
- Diencephalon/cytology
- Diencephalon/growth & development
- Diencephalon/metabolism
- Heparitin Sulfate/metabolism
- Heparitin Sulfate/pharmacology
- Immunohistochemistry
- Lysine/analogs & derivatives*
- Nerve Crush
- Neural Inhibition/physiology
- Optic Nerve/cytology
- Optic Nerve/drug effects
- Optic Nerve/physiology*
- Regeneration/drug effects
- Regeneration/physiology*
- Retinal Ganglion Cells/cytology
- Retinal Ganglion Cells/drug effects
- Retinal Ganglion Cells/metabolism
- Tenascin/metabolism
- Visual Pathways/cytology
- Visual Pathways/drug effects
- Visual Pathways/physiology
- Zebrafish
- PubMed
- 11826114 Full text @ J. Neurosci.
Citation
Becker, C.G. and Becker, T. (2002) Repellent guidance of regenerating optic axons by chondroitin sulfate glycosaminoglycans in zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 22(3):842-853.
Abstract
We analyzed the role of chondroitin sulfate (CS) glycosaminoglycans, putative inhibitors of axonal regeneration in mammals, in the regenerating visual pathway of adult zebrafish. In the adult, CS immunoreactivity was not detectable before or after an optic nerve crush in the optic nerve and tract but was constitutively present in developing and adult nonretinorecipient pretectal brain nuclei, where CSs may form a boundary preventing regenerating optic fibers from growing into these inappropriate locations. Enzymatic removal of CSs by chondroitinase ABC after optic nerve crush significantly increased the number of animals showing erroneous growth of optic axons into the nonretinorecipient magnocellular superficial/posterior pretectal nucleus (83% vs 42% in controls). In vitro, a substrate border of CSs, but not heparan sulfates, strongly repelled regenerating retinal axons from adult zebrafish. We conclude that CSs contribute to repellent axon guidance during regeneration of the optic projection in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping