PUBLICATION
Growth and metabolism of larval zebrafish: effects of swim training
- Authors
- Bagatto, B., Pelster, B., and Burggren, W.W.
- ID
- ZDB-PUB-020219-9
- Date
- 2001
- Source
- The Journal of experimental biology 204(24): 4335-4343 (Journal)
- Registered Authors
- Burggren, Warren, Pelster, Bernd
- Keywords
- development; oxygen consumption; larvae; swimming; hypoxia; zebrafish; Danio rerio
- MeSH Terms
-
- Adaptation, Physiological
- Aging
- Animals
- Larva/growth & development
- Larva/metabolism
- Oxygen/administration & dosage
- Oxygen Consumption
- Swimming/physiology*
- Time Factors
- Yolk Sac/physiology
- Zebrafish/growth & development*
- Zebrafish/metabolism*
- PubMed
- 11815657 Full text @ J. Exp. Biol.
Citation
Bagatto, B., Pelster, B., and Burggren, W.W. (2001) Growth and metabolism of larval zebrafish: effects of swim training. The Journal of experimental biology. 204(24):4335-4343.
Abstract
Larval zebrafish (Danio rerio) of three different age classes ('yolk-sac' larvae, 96 h; 'swim-up' larvae, 9 days old; and 'free-swimming' larvae, 21 days old) were trained for 2, 6 and 11 days, respectively, to swim at 0 body lengths per second (BL s(-1)), 2 BL s(-1) and 5 BL s(-1). Survival was significantly diminished in larvae trained at 5 BL s(-1) compared to controls (0 BL s(-1)). Although training produced no significant differences in mass and length, the youngest larvae absorbed their yolk at a faster rate during training. Routine oxygen consumption ((MO(2)r)) and mass-specific routine oxygen consumption ((MO(2)r,m)) were not significantly affected by chronic training in the yolk-sac larvae and swim-up larvae. However, trained free-swimming larvae had a significantly higher (MO(2)r) (after 11 days of training) and (MO(2)r,m) (after 8 and 11 days of training) compared to control larvae. Trained free-swimming larvae consumed significantly less oxygen during swimming compared to control larvae, as measured by closed-system respirometry. Trained yolk-sac larvae exposed to increasing hypoxia levels were more effective O(2) regulators. Additionally, training enhanced survival during exposure to extreme hypoxia in all age groups. Thus, physiological acclimation to chronic swimming occurs in the earliest stages of life in the zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping