ZFIN ID: ZDB-PUB-020102-3
Novel form of fibronectin from zebrafish mediates infectious hematopoietic necrosis virus infection
Liu, X. and Collodi, P.
Date: 2002
Source: Journal of virology   76(2): 492-498 (Journal)
Registered Authors: Collodi, Paul, Liu, Xiangyu
Keywords: nicotinic acetylcholine receptor, rabies virus, fish rhabdovirus, phosphatidylserine-binding, synthetic peptides, rainbow trout, glycoprotein, protein, embryos, domain
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Cytopathogenic Effect, Viral/drug effects
  • Fibronectins/chemistry*
  • Fibronectins/genetics
  • Fibronectins/metabolism*
  • Fibronectins/pharmacology
  • Gene Expression
  • Infectious hematopoietic necrosis virus/drug effects
  • Infectious hematopoietic necrosis virus/pathogenicity
  • Infectious hematopoietic necrosis virus/physiology*
  • Membrane Proteins/chemistry
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Membrane Proteins/pharmacology
  • Molecular Sequence Data
  • Receptors, Virus/chemistry
  • Receptors, Virus/genetics
  • Receptors, Virus/isolation & purification
  • Receptors, Virus/metabolism
  • Transfection
  • Viral Plaque Assay
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish/virology*
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Zebrafish Proteins/pharmacology
PubMed: 11752139 Full text @ J. Virol.
The presence of a novel form of zebrafish fibronectin (FN2) on the cell surface increased the cell's susceptibility to infection by infectious hematopoietic necrosis virus (IHNV). Unlike other fibronectins, FN2 possesses a truncated structure and accumulates on the cell surface instead of in the extracellular matrix. Fish embryo cells expressing recombinant FN2 were more susceptible to IHNV infection, with a greater percentage of cells exhibiting cytopathic effect (CPE) compared to nontransfected control cells. Incubation of nontransfected cells with soluble recombinant FN2 increased IHNV infection, as measured by plaque assay. The number of plaques increased in correlation with the amount of protein added and the length of time that cells were incubated with the protein. Incubation of IHNV with soluble FN2 before addition to cells also increased infection. FN2 immobilized on the culture surface inhibited IHNV infection. The results indicate that FN2 present on the cell surface is able to mediate IHNV attachment and cell entry.