ZFIN ID: ZDB-PUB-011220-1
Genetic analysis of photoreceptor cell development in the zebrafish retina
Doerre, G. and Malicki, J.
Date: 2002
Source: Mechanisms of Development   110(1-2): 125-138 (Journal)
Registered Authors: Doerre, Geoffrey, Malicki, Jarema
Keywords: Eye; Retina; Photoreceptor; Cell-nonautonomy; Cell¯cell interaction; Zebrafish; Mutant
MeSH Terms:
  • Animals
  • Cell Communication/genetics
  • Cell Death/genetics
  • Microscopy, Electron
  • Mosaicism/genetics
  • Mutation
  • Phenotype
  • Photoreceptor Cells, Vertebrate/cytology*
  • Photoreceptor Cells, Vertebrate/metabolism
  • Retina/cytology
  • Retina/growth & development
  • Retinal Pigments/metabolism
  • Zebrafish/genetics*
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
PubMed: 11744374 Full text @ Mech. Dev.
ABSTRACT
To gain insight into the genetic mechanisms of photoreceptor development, we analyzed a collection of zebrafish mutations characterized by early photoreceptor cell loss. The mutant defects impair outer segment formation and are accompanied by an abnormal distribution of visual pigments. Rods and different cone types display defects of similar severity suggesting that genetic pathways common to all photoreceptors are affected. To investigate whether these phenotypes involve cell-cell interaction defects, we analyzed genetically mosaic animals. Interaction of niezerka photoreceptors with wild-type tissues improves the survival of mutant cells and restores their elongated morphology. In contrast, cells carrying mutations in the loci brudas, elipsa, fleer, and oval retain their defective phenotypes in a wild-type environment indicating cell-autonomy. These experiments identify distinct phenotypic categories of photoreceptor mutants and indicate that zebrafish photo! receptor defects involve both cell-autonomous and cell-nonautonomous mechanisms.
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