PUBLICATION

Genetic analysis of photoreceptor cell development in the zebrafish retina

Authors
Doerre, G. and Malicki, J.
ID
ZDB-PUB-011220-1
Date
2002
Source
Mechanisms of Development   110(1-2): 125-138 (Journal)
Registered Authors
Doerre, Geoffrey, Malicki, Jarema
Keywords
Eye; Retina; Photoreceptor; Cell-nonautonomy; Cell¯cell interaction; Zebrafish; Mutant
MeSH Terms
  • Animals
  • Cell Communication/genetics
  • Cell Death/genetics
  • Microscopy, Electron
  • Mosaicism/genetics
  • Mutation
  • Phenotype
  • Photoreceptor Cells, Vertebrate/cytology*
  • Photoreceptor Cells, Vertebrate/metabolism
  • Retina/cytology
  • Retina/growth & development
  • Retinal Pigments/metabolism
  • Zebrafish/genetics*
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
PubMed
11744374 Full text @ Mech. Dev.
Abstract
To gain insight into the genetic mechanisms of photoreceptor development, we analyzed a collection of zebrafish mutations characterized by early photoreceptor cell loss. The mutant defects impair outer segment formation and are accompanied by an abnormal distribution of visual pigments. Rods and different cone types display defects of similar severity suggesting that genetic pathways common to all photoreceptors are affected. To investigate whether these phenotypes involve cell-cell interaction defects, we analyzed genetically mosaic animals. Interaction of niezerka photoreceptors with wild-type tissues improves the survival of mutant cells and restores their elongated morphology. In contrast, cells carrying mutations in the loci brudas, elipsa, fleer, and oval retain their defective phenotypes in a wild-type environment indicating cell-autonomy. These experiments identify distinct phenotypic categories of photoreceptor mutants and indicate that zebrafish photo! receptor defects involve both cell-autonomous and cell-nonautonomous mechanisms.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping