PUBLICATION

Comparative anatomy of the histaminergic and other aminergic systems in zebrafish (Danio rerio)

Authors
Kaslin, J. and Panula, P.
ID
ZDB-PUB-011204-2
Date
2001
Source
The Journal of comparative neurology   440(4): 342-377 (Journal)
Registered Authors
Kaslin, Jan, Panula, Pertti
Keywords
dopamine; noradrenaline; serotonin; raphe nucleus; locus coeruleus; tuberomammillary nucleus
MeSH Terms
  • Animals
  • Biogenic Monoamines/analysis*
  • Brain/cytology*
  • Brain Chemistry*
  • Dopa Decarboxylase/analysis
  • Dopamine/analysis
  • Female
  • Histamine/analysis
  • Hypothalamic Area, Lateral/cytology
  • Hypothalamus/cytology
  • Immunohistochemistry
  • Locus Coeruleus/cytology
  • Male
  • Neurons/chemistry
  • Neurons/enzymology
  • Raphe Nuclei/cytology
  • Rhombencephalon/cytology
  • Serotonin/analysis
  • Superior Colliculi/cytology
  • Thalamus/cytology
  • Tyrosine 3-Monooxygenase/analysis
  • Zebrafish/anatomy & histology*
PubMed
11745628 Full text @ J. Comp. Neurol.
Abstract
The histaminergic system and its relationships to the other aminergic transmitter systems in the brain of the zebrafish were studied by using confocal microscopy and immunohistochemistry on brain whole-mounts and sections. All monoaminergic systems displayed extensive, widespread fiber systems that innervated all major brain areas, often in a complementary manner. The ventrocaudal hypothalamus contained all monoamine neurons except noradrenaline cells. Histamine (HA), tyrosine hydroxylase (TH), and serotonin (5-HT) -containing neurons were all found around the posterior recess (PR) of the caudal hypothalamus. TH- and 5-HT-containing neurons were found in the periventricular cell layer of PR, whereas the HA-containing neurons were in the surrounding cell layer as a distinct boundary. Histaminergic neurons, which send widespread ascending and descending fibers, were all confined to the ventrocaudal hypothalamus. Histaminergic neurons were medium in size (~12μm) with varicose ascending and descending ipsilateral and contralateral fiber projections. Histamine was stored in vesicles in two types of neurons and fibers. A close relationship between HA fibers and serotonergic raphe neurons and noradrenergic locus coeruleus neurons was evident. Putative synaptic contacts were occasionally detected between HA and TH or 5-HT neurons. These results indicate that reciprocal contacts between monoaminergic systems are abundant and complex. The results also provide evidence of homologies to mammalian systems and allow identification of several previously uncharacterized systems in zebrafish mutants.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping