PUBLICATION

Direct imaging of in vivo neuronal migration in the developing cerebellum

Authors
Köster, R.W. and Fraser, S.E.
ID
ZDB-PUB-011203-4
Date
2001
Source
Current biology : CB   11(23): 1858-1863 (Journal)
Registered Authors
Fraser, Scott E., Köster, Reinhard W.
Keywords
central nervous system, rhombic lip, zebrafish, expression, induction, netrin 1, embryos, origin
MeSH Terms
  • Animals
  • Cell Movement*
  • Cerebellum/cytology*
  • Cerebellum/embryology
  • Neurons/cytology*
  • Zebrafish/embryology
PubMed
11728308 Full text @ Curr. Biol.
Abstract
The upper rhombic lip (URL), a germinal zone in the dorsoanterior hindbrain, has long been known to be a source for neurons of the vertebrate cerebellum. It was thought to give rise to dorsally migrating granule cell precursors (Figure 1e); however, recent fate mapping studies have questioned the exclusive contributions of the URL to granule cells. By taking advantage of the clarity of the zebrafish embryo during the stages of brain morphogenesis, we have followed the fate of neuronal precursor cells generated within the upper rhombic lip directly. Combining a novel GFP labeling strategy with in vivo time-lapse imaging, we find, contrary to the former view, that most URL-descendants migrate anterior toward the midhindbrain boundary (MHB) and then course ventrally along the MHB (Figure 1f). As the migrating neuronal precursors reach the MHB, they form ventrally extending projections, likely axons, and continue ventral migration to settle outside of the cerebellum, in the region of the ventral brainstem. Thus, we define a new pathway for URL-derived neuronal precursor cells consistent with the recent fate maps. In addition, our results strongly suggest that the MHB plays a crucial role, not only in induction and patterning of the cerebellar anlage, but also in organizing its later morphogenesis by influencing cell migration.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping