PUBLICATION
Reporter gene expression in fish following cutaneous infection with pantropic retroviral vectors
- Authors
- Paul, T.A., Burns, J.C., Shike, H., Getchell, R., Bowser, P.R., Whitlock, K.E., and Casey, J.W.
- ID
- ZDB-PUB-010918-3
- Date
- 2001
- Source
- Marine biotechnology (New York, N.Y.) 3: S81-S87 (Journal)
- Registered Authors
- Bowser, Paul R., Burns, Jane C., Paul, Thomas A., Whitlock, Kate
- Keywords
- retroviral vector, promoter, experimental infection, zebrafish, walleye dermal sarcoma virus, dermal sarcoma, stizostedion vitreum, skin tumors, high titer, cells, generation, embryos, cyclin, rerio
- MeSH Terms
- none
- PubMed
- none
Citation
Paul, T.A., Burns, J.C., Shike, H., Getchell, R., Bowser, P.R., Whitlock, K.E., and Casey, J.W. (2001) Reporter gene expression in fish following cutaneous infection with pantropic retroviral vectors. Marine biotechnology (New York, N.Y.). 3:S81-S87.
Abstract
A central issue in gene delivery systems is choosing promoters that will direct defined and sustainable levels of gene expression. Pantropic retroviral vectors provide a means to insert genes into either somatic or germline cells. In this study, we focused on somatic cell infection by evaluating the activity of 3 promoters inserted by vectors into fish cell lines and fish skin using pantropic retroviruses. In bluegill and zebrafish cell lines, the highest levels of luciferase expression were observed from the 5 ' murine leukemia virus long terminal repeat of the retroviral vector. The Rous sarcoma virus long terminal repeat and cytomegalovirus early promoter, as internal promoters, generated lower levels of luciferase. Luciferase reporter vectors infected zebrafish skin, as measured by the presence of viral DNA, and expressed luciferase. We infected developing walleye dermal sarcomas with retroviral vectors to provide an environment with enhanced cell proliferation, a condition necessary for integration of the provirus into the host genome. We demonstrated a 4-fold to 7-fold increase in luciferase gene expression in tumor tissue over infections in normal walleye skin.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping