ZFIN ID: ZDB-PUB-010912-26
Caged Q-rhodamine dextran: a new photoactivated fluorescent tracer
Gee, K.R., Weinberg, E.S., and Kozlowski, D.J.
Date: 2001
Source: Bioorganic & medicinal chemistry letters   11(16): 2181-2183 (Journal)
Registered Authors: Kozlowski, David J., Weinberg, Eric
Keywords: none
MeSH Terms:
  • Animals
  • Dextrans/chemical synthesis*
  • Dextrans/chemistry
  • Embryo, Nonmammalian
  • Fluorescent Dyes/chemical synthesis*
  • Fluorescent Dyes/chemistry
  • Microscopy, Fluorescence
  • Photochemistry
  • Rhodamines/chemical synthesis*
  • Rhodamines/chemistry
  • Zebrafish
PubMed: 11514165 Full text @ Bioorg. Med. Chem. Lett.
An amine-reactive caged rhodamine was synthesized and conjugated to aminodextran. The resulting tracer was injected into a single cell zebrafish embryo, and a portion of the tracer was photolyzed in a single cell after development. The resulting fluorescent cell was imaged by fluorescence microscopy through a single round of cell division.