PUBLICATION
Stress-induced apoptosis by heat shock, UV and gamma-ray irradiation in zebrafish embryos detected by increased caspase activity and whole-mount TUNEL staining
- Authors
- Yabu, T., Todoriki, S., and Yamashita, M.
- ID
- ZDB-PUB-010718-17
- Date
- 2001
- Source
- Fisheries science : FS 67(2): 333-340 (Journal)
- Registered Authors
- Yamashita, Michiaki
- Keywords
- apoptosis, caspase, Danio rerio, embryo, heat shook, UV, zebrafish, gamma-ray irradiation, cell-death, ICE/CED-3 protease, identification, gastrulation, endonuclease, expression, mechanism, induction, family
- MeSH Terms
- none
- PubMed
- none
Citation
Yabu, T., Todoriki, S., and Yamashita, M. (2001) Stress-induced apoptosis by heat shock, UV and gamma-ray irradiation in zebrafish embryos detected by increased caspase activity and whole-mount TUNEL staining. Fisheries science : FS. 67(2):333-340.
Abstract
Environmental stress-induced apoptosis in zebrafish Danio rerio embryos was characterized by assaying caspase-3-like activity and whole-mount terminal deoxynucleotidyl nick-end labeling (TUNEL) staining. Severe stress conditions, such as heat shock at 39 degreesC for 1 h, ultraviolet light at 10-100 mJ/cm(2) and gamma -ray irradiation at 5-20 Gy induced extensive apoptosis in embryos. Apoptotic cells were observed after the bud and 1-somite stages in normal embryos by TUNEL staining, and after stress treatment many TUNEL-positive cells were found in the enveloping and deep cell layers and the larval fin. The caspase-3-like activity increased severalfold during stress-induced apoptosis in a dose-dependent manner. These findings indicate that apoptotic pathways, mediated by caspase-3-like activity, play a major role in zebrafish embryogenesis under stress conditions.
http://www.blackwell-synergy.com/Journals/content/abstracts/fis/2001/67/2/abstract_fis233.asp?journal=fis&issueid=5417&artid=103375&cid=fis.2001.1&ftype=abstracts&Inst=true
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
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