PUBLICATION
Estrogen and xenoestrogens upregulate the brain aromatase isoform (P450aromB) and perturb markers of early development in zebrafish (Danio rerio)
- Authors
- Kishida, M., McLellan, M., Miranda, J.A., and Callard, G.V.
- ID
- ZDB-PUB-010706-12
- Date
- 2001
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 129(2-3): 261-268 (Journal)
- Registered Authors
- Callard, Gloria V., Kishida, Mitsuyo
- Keywords
- brain aromatase; estrogen; xenoestrogen; neurodevelopment in zebrafish
- MeSH Terms
-
- Animals
- Aromatase/genetics
- Aromatase/metabolism*
- Biomarkers/analysis
- Brain/drug effects
- Brain/enzymology
- Brain/metabolism*
- Dose-Response Relationship, Drug
- Enzyme Induction/drug effects
- Estradiol/agonists
- Estradiol/pharmacology
- Estrogens/agonists
- Estrogens/pharmacology*
- Isoenzymes/genetics
- Isoenzymes/metabolism
- Microscopy, Phase-Contrast
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
- Zebrafish/embryology*
- Zebrafish/metabolism
- PubMed
- 11399458 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
- CTD
- 11399458
Citation
Kishida, M., McLellan, M., Miranda, J.A., and Callard, G.V. (2001) Estrogen and xenoestrogens upregulate the brain aromatase isoform (P450aromB) and perturb markers of early development in zebrafish (Danio rerio). Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 129(2-3):261-268.
Abstract
Estrogen synthesized in the brain itself by the action of cytochrome P450 aromatase (P450arom) is known to have permanent organizing effects on the developing CNS. In fish, estrogen upregulates the predominant brain isoform (P450aromB), implying that xenoestrogens (XE) could act as neurodevelopmental toxicants by altering P450aromB. To test this hypothesis, zebrafish embryos were exposed to 17beta-estradiol (E(2)), diethylstilbestrol (DES, a potent agonist), and bisphenol A (BPA, a weak agonist). RT-PCR/Southern transfer analysis showed that E(2) (0.01-10 μM) upregulated P450aromB in a dose-response manner. The effect of DES (0.01 μM) was similar to 1 μM E(2) (three- to four-fold higher than control), but BPA was less effective (
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping