ZFIN ID: ZDB-PUB-010601-3
Analysis of pancreatic development in living transgenic zebrafish embryos
Huang, H., Vogel, S.S., Liu, N., Melton, D.A., and Lin, S.
Date: 2001
Source: Molecular and Cellular Endocrinology   177(1-2): 117-124 (Journal)
Registered Authors: Huang, Haigen, Lin, Shuo, Liu, Ningai, Melton, Douglas A.
Keywords: zebrafish; pancreas; Pdx-1; insulin; transgenic
MeSH Terms:
  • 5' Untranslated Regions
  • Animals
  • Animals, Genetically Modified/embryology
  • Animals, Genetically Modified/genetics
  • Animals, Genetically Modified/metabolism
  • Embryo, Nonmammalian/anatomy & histology
  • Embryo, Nonmammalian/chemistry
  • Green Fluorescent Proteins
  • Homeodomain Proteins*
  • Insulin/genetics
  • Insulin/metabolism
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • Organ Specificity
  • Pancreas/embryology
  • Pancreas/growth & development*
  • Time Factors
  • Trans-Activators/metabolism
  • Zebrafish/embryology*
PubMed: 11377827 Full text @ Mol. Cell. Endocrinol.
Using DNA constructs containing regulatory sequences of the zebrafish Pdx-1 and insulin genes, germline transgenic zebrafish expressing the green fluorescent protein (GFP) reporter gene in the pancreas were generated. For both constructs, the GFP expression patterns in transgenic embryos were consistent with the mRNA expression patterns detected by RNA in situ hybridization. A deletion promoter analysis revealed that positive and negative cis-acting elements were involved in regulation of insulin gene expression. Three-dimensional reconstructions imaged from living embryos using two-photon laser-scanning microscopy (TPLSM) demonstrated that the zebrafish pancreas is formed from a single dorsal pancreatic cell mass. This is in contrast to mammals where the pancreas derives from both dorsal and ventral anlage. These transgenic fish should be useful for in vivo studies of factors involved in specifying and regulating pancreatic development and function.