PUBLICATION
Overexpression of a zebrafish ARNT2-like factor represses CYP1A transcription in ZLE cells
- Authors
- Wang, W.D., Wu, J.C., Hsu, H.J., Kong, Z.L., and Hu, C.H.
- ID
- ZDB-PUB-010426-1
- Date
- 2000
- Source
- Marine biotechnology (New York, N.Y.) 2(4): 376-386 (Journal)
- Registered Authors
- Hu, Chin-Hwa, Kong, Richard Zwe-Ling
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 10960127 Full text @ Mar. Biotechnol.
Citation
Wang, W.D., Wu, J.C., Hsu, H.J., Kong, Z.L., and Hu, C.H. (2000) Overexpression of a zebrafish ARNT2-like factor represses CYP1A transcription in ZLE cells. Marine biotechnology (New York, N.Y.). 2(4):376-386.
Abstract
Aryl hydrocarbon receptor nuclear translocator (ARNT) factors belong to a novel basic-helix-loop-helix-PAS (bHLH-PAS) transcription factor family that controls a variety of physiological and developmental processes. In a previous study, we obtained a partial complementary DNA fragment of an ARNT2-like factor from zebrafish embryo, liver, and other tissues by reverse transcription-polymerase chain reaction. In an effort to characterize the function of this factor, we screened an embryonic complementary DNA library and obtained a complete cDNA of this ARNT2-like factor, zARNT2A. The deduced protein sequence of zARNT2A encompasses the basic-helix-loop-helix and PAS-A/B motifs and shares highest sequence similarity with the amino-terminal half of mouse ARNT2 factor. However, it lacks a carboxy-terminal transactivation motif following the PAS-A/B motifs. Transient expression of zARNT2A in cultured cells resulted in repression of TCDD-dependent CYP1A transcription. Whole-mount in situ hybridization revealed that zARNT2A is expressed in brain and pronephros at prime-5 stages. In adult fish, zARNT2A messenger RNA is transcribed in a wide range of tissues, which indicates that zARNT2A and its corresponding signal transduction mechanisms have important roles in fish development and other physiological aspects.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping