ZFIN ID: ZDB-PUB-010423-3
astray, a zebrafish roundabout homolog required for retinal axon guidance
Fricke, C., Lee, J.S., Geiger-Rudolph, S., Bonhoeffer, F., and Chien, C.-B.
Date: 2001
Source: Science (New York, N.Y.)   292(5516): 507-510 (Journal)
Registered Authors: Bonhoeffer, Friedrich, Chien, Chi-Bin, Fricke, Cornelia, Geiger-Rudolph, Silke, Lee, Jeong-Soo
Keywords: none
MeSH Terms:
  • Alleles
  • Animals
  • Axons/physiology*
  • Body Patterning
  • Chromosome Mapping
  • Crosses, Genetic
  • Eye/embryology
  • Eye/transplantation
  • Female
  • Gene Expression Regulation, Developmental
  • Genes
  • In Situ Hybridization
  • Male
  • Mutation
  • Nerve Tissue Proteins/genetics*
  • Nerve Tissue Proteins/physiology
  • Phenotype
  • Receptors, Immunologic/genetics*
  • Receptors, Immunologic/physiology*
  • Retina/embryology
  • Retina/metabolism
  • Retinal Ganglion Cells/metabolism
  • Retinal Ganglion Cells/physiology*
  • Superior Colliculi/cytology
  • Superior Colliculi/embryology*
  • Visual Pathways/embryology
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish Proteins
PubMed: 11313496 Full text @ Science
As growing retinotectal axons navigate from the eye to the tectum, they sense guidance molecules distributed along the optic pathway. Mutations in the zebrafish astray gene severely disrupt retinal axon guidance, causing anterior-posterior pathfinding defects, excessive midline crossing, and defasciculation of the retinal projection. Eye transplantation experiments show that astray function is required in the eye. We identify astray as zebrafish robo2, a member of the Roundabout family of axon guidance receptors. Retinal ganglion cells express robo2 as they extend axons. Thus, robo2 is required for multiple axon guidance decisions during establishment of the vertebrate visual projection