PUBLICATION
Identification and characterization of a mammalian enzyme catalyzing the asymmetric oxidative cleavage of provitamin A
- Authors
- Kiefer, C., Hessel, S., Lampert, J.M., Vogt, K., Lederer, M.O., Breithaupt, D.E., and von Lintig, J.
- ID
- ZDB-PUB-010417-11
- Date
- 2001
- Source
- The Journal of biological chemistry 276(17): 14110-14116 (Journal)
- Registered Authors
- Lampert, Johanna
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Carotenoids/chemistry
- Carotenoids/metabolism
- Catalysis
- Chromatography, High Pressure Liquid
- Cloning, Molecular
- DNA, Complementary/metabolism
- Drosophila/enzymology
- Drosophila Proteins
- Expressed Sequence Tags
- Female
- Gene Library
- Humans
- Male
- Mass Spectrometry
- Mice
- Mice, Inbred BALB C
- Models, Chemical
- Molecular Sequence Data
- Norisoprenoids*
- Oxygen/metabolism*
- Oxygenases/chemistry*
- Oxygenases/metabolism
- Phenotype
- Phylogeny
- RNA/metabolism
- Retinaldehyde/chemistry
- Sequence Homology, Amino Acid
- Terpenes/chemistry
- Time Factors
- Tissue Distribution
- Vitamin A/chemistry
- Vitamin A/metabolism*
- Zebrafish
- beta Carotene/chemistry*
- beta Carotene/metabolism
- beta-Carotene 15,15'-Monooxygenase
- PubMed
- 11278918 Full text @ J. Biol. Chem.
Citation
Kiefer, C., Hessel, S., Lampert, J.M., Vogt, K., Lederer, M.O., Breithaupt, D.E., and von Lintig, J. (2001) Identification and characterization of a mammalian enzyme catalyzing the asymmetric oxidative cleavage of provitamin A. The Journal of biological chemistry. 276(17):14110-14116.
Abstract
In vertebrates, symmetric versus asymmetric cleavage of b-carotene in the biosynthesis of vitamin A and its derivatives has been controversially discussed. Recently we have been able to identify a cDNA encoding a metazoan b,b-carotene-15,15'-dioxygenase from the fruit fly Drosophila melanogaster. This enzyme catalyzes the key step in vitamin A biosynthesis, symmetrically cleaving b-carotene to give two molecules of retinal. Mutations in the corresponding gene are known to lead to a blind, vitamin A deficient phenotype. Orthologs of this enzyme have very recently also been found in vertebrates and molecularly characterized. Here we report the identification of a cDNA from mouse encoding a second type of carotene dioxygenase catalyzing exclusively the asymmetric oxidative cleavage of b-carotene at the 9',10' double bond of b-carotene and resulting in the formation of b-apo-10'-carotenal and b-ionone, a substance known as a floral scent from, e.g., roses. Besides b-carotene, lycopene is also oxidatively cleaved by the enzyme. The deduced amino acid sequence shares significant sequence identity with the b,b-carotene-15,15'-dioxygenases and the two enzyme types have several conserved motifs. To establish its occurrence in different vertebrates, we then attempted and succeeded in cloning cDNAs encoding this new type of carotene dioxygenase from human and zebrafish as well. As regards their possible role, the apocarotenals formed by this enzyme may be the precursors for the biosynthesis of retinoic acid or exert unknown physiological effects. Thus, in contrast to Drosophila, in vertebrates both symmetric and asymmetric cleavage pathways exist for carotenes, revealing a greater complexity of carotene metabolism here.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping