|ZFIN ID: ZDB-PUB-010220-9|
Molecular structure, dynamic expression, and promoter analysis of zebrafish (Danio rerio) myf-5 gene
Chen, Y.-H., Lee, W.-C., Liu, C.-F., and Tsai, H.-J.
|Source:||Genesis (New York, N.Y. : 2000) 29(1): 22-35 (Journal)|
|Registered Authors:||Chen, Yau-Hung, Lee, Wen-Chih, Tsai, Huai-Jen|
|Keywords:||fish; microinjection; molecular structure; muscle-specificity; somitogenesis; transgenic fish|
|PubMed:||11135459 Full text @ Genesis|
Chen, Y.-H., Lee, W.-C., Liu, C.-F., and Tsai, H.-J. (2001) Molecular structure, dynamic expression, and promoter analysis of zebrafish (Danio rerio) myf-5 gene. Genesis (New York, N.Y. : 2000). 29(1):22-35.
ABSTRACTSummary: We isolated a 1,438 bp cDNA fragment that encoded Myf-5 myogenic factor of zebrafish. The deduced amino acid contained 237 residues, including the basic helix-loop-helix domain that is conserved in all known Myf-5. The zebrafish myf-5 transcripts were first detectable at 7.5 hpf, increased substantially until 16 hpf, and then declined gradually to an undetectable level by 26 hpf. During somitogenesis, zebrafish myf-5 transcripts were distributed mainly in the somites and segmental plates. Prominent signals occurred transiently in adaxial cells in two parallel rows but did not extend beyond the positive-signal somites. Various lengths of upstream region of zebrafish myf-5 fused with EGFP gene were used to carry out transgenic analysis. Results showed that a small, 82 bp (nucleotide positions from -82 to -1), regulatory cassette is sufficient to control the somite- and stage-specific expression of zebrafish myf-5 during early development. genesis 29:22-35, 2001.