ZFIN ID: ZDB-PUB-010220-9
Molecular structure, dynamic expression, and promoter analysis of zebrafish (Danio rerio) myf-5 gene
Chen, Y.-H., Lee, W.-C., Liu, C.-F., and Tsai, H.-J.
Date: 2001
Source: Genesis (New York, N.Y. : 2000)   29(1): 22-35 (Journal)
Registered Authors: Chen, Yau-Hung, Lee, Wen-Chih, Tsai, Huai-Jen
Keywords: fish; microinjection; molecular structure; muscle-specificity; somitogenesis; transgenic fish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers/chemistry
  • DNA-Binding Proteins*
  • Exons
  • Gene Expression
  • Genes, Regulator
  • Genetic Vectors
  • Green Fluorescent Proteins
  • In Situ Hybridization
  • Introns
  • Luminescent Proteins/metabolism
  • Molecular Sequence Data
  • Muscle Proteins/genetics*
  • Muscle Proteins/metabolism
  • Myogenic Regulatory Factor 5
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic*
  • RNA, Messenger/biosynthesis
  • Sequence Homology, Amino Acid
  • Trans-Activators*
  • Transcription Factors/genetics*
  • Transcription, Genetic
  • Zebrafish/genetics*
PubMed: 11135459 Full text @ Genesis
ABSTRACT
Summary: We isolated a 1,438 bp cDNA fragment that encoded Myf-5 myogenic factor of zebrafish. The deduced amino acid contained 237 residues, including the basic helix-loop-helix domain that is conserved in all known Myf-5. The zebrafish myf-5 transcripts were first detectable at 7.5 hpf, increased substantially until 16 hpf, and then declined gradually to an undetectable level by 26 hpf. During somitogenesis, zebrafish myf-5 transcripts were distributed mainly in the somites and segmental plates. Prominent signals occurred transiently in adaxial cells in two parallel rows but did not extend beyond the positive-signal somites. Various lengths of upstream region of zebrafish myf-5 fused with EGFP gene were used to carry out transgenic analysis. Results showed that a small, 82 bp (nucleotide positions from -82 to -1), regulatory cassette is sufficient to control the somite- and stage-specific expression of zebrafish myf-5 during early development. genesis 29:22-35, 2001.
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