PUBLICATION
Topographic restriction of TAG-1 expression in the developing retinotectal pathway and target dependent reexpression during axon regeneration
- Authors
- Lang, D.M., Warren, J.T., Klisa, C., and Stürmer, C.A.
- ID
- ZDB-PUB-010219-6
- Date
- 2001
- Source
- Molecular and cellular neurosciences 17(2): 398-414 (Journal)
- Registered Authors
- Klisa, Christiane, Lang, Dirk, Stuermer, Claudia, Warren, James T., Jr.
- Keywords
- none
- MeSH Terms
-
- Animals
- Axons/chemistry
- Axons/physiology*
- Cell Adhesion Molecules, Neuronal*
- Contactin 2
- Gene Expression Regulation, Developmental
- Goldfish
- Membrane Glycoproteins/analysis
- Membrane Glycoproteins/genetics*
- Nerve Regeneration/physiology*
- Optic Nerve/cytology
- Optic Nerve/embryology
- Optic Nerve/physiology
- Optic Nerve Injuries
- RNA, Messenger/analysis
- Retinal Ganglion Cells/chemistry
- Retinal Ganglion Cells/cytology
- Retinal Ganglion Cells/physiology*
- Superior Colliculi/cytology
- Superior Colliculi/embryology
- Zebrafish
- PubMed
- 11178876 Full text @ Mol. Cell Neurosci.
Citation
Lang, D.M., Warren, J.T., Klisa, C., and Stürmer, C.A. (2001) Topographic restriction of TAG-1 expression in the developing retinotectal pathway and target dependent reexpression during axon regeneration. Molecular and cellular neurosciences. 17(2):398-414.
Abstract
TAG-1, a glycosylphosphatidyl inositol (GPI)-anchored protein of the immunoglobulin (Ig) superfamily, exhibits an unusual spatiotemporal expression pattern in the fish visual pathway. Using in situ hybridization and new antibodies (Abs) against fish TAG-1 we show that TAG-1 mRNA and anti-TAG-1 staining is restricted to nasal retinal ganglion cells (RGCs) in 24- to 72-h-old zebrafish embryos and in the adult, continuously growing goldfish retina. Anti-TAG-1 Abs selectively label nasal RGC axons in the nerve, optic tract, and tectum. Axotomized RGCs reexpress TAG-1, which occurs as late as 12 days after optic nerve lesion, when regenerating RGC axons arrive in the tectum, suggesting TAG-1 reexpression is target contact-dependent. Accordingly, TAG-1 reexpression ceases upon interruption of the regenerating projection by a second lesion. The topographic restriction of TAG-1 expression and its target dependency during regeneration suggests that TAG-1 might play a role in the retinotopic organization and restoration of the retinotectal pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping