PUBLICATION
Functional analysis of zebrafish GDNF
- Authors
- Shepherd, I.T., Beattie, C.E., and Raible, D.W.
- ID
- ZDB-PUB-010219-1
- Date
- 2001
- Source
- Developmental Biology 231(2): 420-435 (Journal)
- Registered Authors
- Beattie, Christine, Raible, David, Shepherd, Iain T.
- Keywords
- zebrafish; GDNF; GFR1; c-ret; kidney; ENS; primary motor neuron; morpholino
- MeSH Terms
-
- Axons/metabolism
- Axons/physiology
- Oligonucleotides, Antisense/metabolism
- In Situ Hybridization
- Ligands
- Sequence Homology, Amino Acid
- Enteric Nervous System/metabolism
- Motor Neurons/metabolism
- Open Reading Frames
- Proto-Oncogene Proteins c-ret
- Chromosome Mapping
- Kidney/embryology
- Kidney/metabolism
- Glial Cell Line-Derived Neurotrophic Factor Receptors
- Amino Acid Sequence
- Proto-Oncogene Proteins/metabolism
- Proto-Oncogene Proteins/physiology
- Nerve Tissue Proteins/chemistry*
- Nerve Tissue Proteins/genetics*
- Spinal Cord/metabolism
- RNA, Messenger/metabolism
- Promoter Regions, Genetic
- Drosophila Proteins*
- Zebrafish
- Glial Cell Line-Derived Neurotrophic Factor
- Animals
- Somites/metabolism
- Nerve Growth Factors*
- Zebrafish Proteins
- Receptor Protein-Tyrosine Kinases/metabolism
- Receptor Protein-Tyrosine Kinases/physiology
- Molecular Sequence Data
- PubMed
- 11237470 Full text @ Dev. Biol.
Citation
Shepherd, I.T., Beattie, C.E., and Raible, D.W. (2001) Functional analysis of zebrafish GDNF. Developmental Biology. 231(2):420-435.
Abstract
We have identified zebrafish orthologues of glial cell line-derived neurotrophic factor (GDNF) and the ligand-binding component of its receptor GFR1. We examined the mRNA expression pattern of these genes in the developing spinal cord primary motor neurons (PMN), kidney, and enteric nervous systems (ENS) and have identified areas of correlated expression of the ligand and the receptor that suggest functional significance. Many aspects of zebrafish GDNF expression appear conserved with those reported in mouse, rat, and avian systems. In the zebrafish PMN, GFR1 is only expressed in the CaP motor neuron while GDNF is expressed in the ventral somitic muscle that it innervates. To test the functional significance of this correlated expression pattern, we ectopically overexpressed GDNF in somitic muscle during the period of motor axon outgrowth and found specific perturbations in the pattern of CaP axon growth. We also depleted GDNF protein in zebrafish embryos using morpholino antisense oligos and found that GDNF protein is critical for the development of the zebrafish ENS but appears dispensable for the development of the kidney and PMN.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping