PUBLICATION

Dispersion of cyclin B mRNA aggregation is coupled with translational activation of the mRNA during zebrafish oocyte maturation

Authors
Kondo, T., Kotani, T., and Yamashita, M.
ID
ZDB-PUB-010131-31
Date
2001
Source
Developmental Biology   229(2): 421-431 (Journal)
Registered Authors
Kotani, Tomoya
Keywords
Cdc2; cyclin B; cytochalasin B; meiosis reinitiation; microfilaments; MPF; mRNA; oocyte maturation; translational control; zebrafish
MeSH Terms
  • Animals
  • CDC2 Protein Kinase/metabolism
  • Cyclin B/genetics*
  • Cytochalasin B/pharmacology
  • Female
  • Gene Expression Regulation, Developmental
  • Hydroxyprogesterones/pharmacology
  • Oocytes/drug effects
  • Oocytes/physiology*
  • Protein Biosynthesis*/drug effects
  • RNA, Messenger/drug effects
  • RNA, Messenger/metabolism*
  • Transcription, Genetic*
  • Zebrafish
PubMed
11150242 Full text @ Dev. Biol.
Abstract
Cyclin B mRNA stored in immature zebrafish oocytes is translationally activated upon the stimulation of 17alpha,20beta-dihydroxy-4-pregnen-3-one (17alpha,20beta-DP), an event prerequisite for initiating oocyte maturation in this species. We investigated localization of cyclin B mRNA in zebrafish oocytes. Cyclin B mRNA was found to be exclusively localized as an aggregation along the cytoplasm at the animal pole of full-grown immature oocytes. When oocytes were treated with 17alpha,20beta-DP, a meshwork of microfilaments in the oocyte cortex disappeared and the aggregation of cyclin B mRNA dispersed just prior to the initiation of cyclin B synthesis and germinal vesicle breakdown (GVBD). Cytochalasin B, but not nocodazole or taxol, deformed the aggregation of cyclin B mRNA, indicating the involvement of microfilaments in organizing this form. Like 17alpha,20beta-DP, cytochalasin B (10 mug/ml) induced both complete dispersion of the aggregation and translational activation of cyclin B mRNA, forcing the oocytes to undergo GVBD without 17alpha,20beta-DP. Conversely, disturbance of the aggregation of cyclin B mRNA with a low concentration (1 mug/ml) of cytochalasin B inhibited 17alpha,20beta-DP-induced GVBD. These results suggest that the direct change in cyclin B mRNA from the aggregated form to the dispersed form is responsible for translational activation of the mRNA during zebrafish oocyte maturation.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes