PUBLICATION
Characterization of a zebrafish (Danio rerio) sphingosine 1-phosphate receptor expressed in the embryonic brain
- Authors
- Im, D.S., Ungar, A.R., and Lynch, K.R.
- ID
- ZDB-PUB-001221-12
- Date
- 2000
- Source
- Biochemical and Biophysical Research Communications 279(1): 139-143 (Journal)
- Registered Authors
- Ungar, Anne
- Keywords
- zebrafish; Edg1; sphingosine 1-phosphate; G protein-coupled receptor; signal transduction; evolution
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Brain/metabolism*
- Cloning, Molecular
- DNA Primers
- Humans
- Molecular Sequence Data
- Receptors, Cell Surface/metabolism*
- Receptors, G-Protein-Coupled*
- Receptors, Lysophospholipid
- Sequence Homology, Amino Acid
- Zebrafish/embryology*
- PubMed
- 11112429 Full text @ Biochem. Biophys. Res. Commun.
Citation
Im, D.S., Ungar, A.R., and Lynch, K.R. (2000) Characterization of a zebrafish (Danio rerio) sphingosine 1-phosphate receptor expressed in the embryonic brain. Biochemical and Biophysical Research Communications. 279(1):139-143.
Abstract
Sphingosine 1-phosphate elicits a variety of responses in mammals via at least five G protein-coupled Edg receptors. We cloned zebrafish edg1 and expressed it in Rh7777 cells. In these cultures, S1P inhibited forskolin-driven rises in cAMP and this response was eliminated by pretreatment of the cultures with pertussis toxin. In Rh7777 membranes, S1P stimulated GTPgamma[(35)S] binding 2-3 fold. Zebrafish edg1 is expressed in embryonic brain, particularly ventral diencephalon, optic stalks, and anterior hindbrain. Our findings suggest that nonmammalian vertebrates use S1P to signal during embryogenesis and that the properties of Edg1 receptor have been conserved for 400 million years.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping