PUBLICATION
Transient nuclear localization of Fyn kinase during development in zebrafish
- Authors
- Rongish, B.J. and Kinsey, W.H.
- ID
- ZDB-PUB-001026-1
- Date
- 2000
- Source
- The Anatomical record 260(2): 115-123 (Journal)
- Registered Authors
- Kinsey, William H.
- Keywords
- tyrosine; Src; nucleus; embryo
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Antibodies
- Antigen-Antibody Complex/analysis
- Biological Transport/physiology
- Cell Nucleus/enzymology*
- Cytosol/enzymology
- Embryo, Nonmammalian/enzymology
- Gastrula/enzymology
- Gene Expression Regulation, Developmental
- Gene Library
- Molecular Sequence Data
- Precipitin Tests
- Proto-Oncogene Proteins/genetics*
- Proto-Oncogene Proteins/immunology
- Proto-Oncogene Proteins/metabolism*
- Proto-Oncogene Proteins c-fyn
- RNA, Messenger/analysis
- Signal Transduction/physiology
- Zebrafish
- Zebrafish Proteins
- src Homology Domains/genetics
- PubMed
- 10993948 Full text @ Anat. Rec.
Citation
Rongish, B.J. and Kinsey, W.H. (2000) Transient nuclear localization of Fyn kinase during development in zebrafish. The Anatomical record. 260(2):115-123.
Abstract
Fyn protein tyrosine kinase is present in the unfertilized and fertilized egg, becomes activated within minutes following fertilization, and has been localized to the cortical cytoplasm and spindle apparatus of the zygote. In order to establish the expression pattern of Fyn in the early embryo, we examined the distribution pattern of Fyn by immunofluorescence microscopy. Fyn protein is distributed evenly among cells of the cleavage stage zebrafish embryo and is concentrated in the cortical region of each cell. During blastula and gastrula stages, Fyn was expressed in all cells, however a subpopulation of cells exhibited strong nuclear staining for Fyn. Nuclear Fyn staining was not observed after the gastrula period of development, nor in the adult zebrafish. Immunoprecipitation of Fyn from isolated mid-blastula nuclei confirmed Fyn was present in the nucleus. This is, to our knowledge, the first demonstration of Fyn kinase, which lacks a nuclear localization signal, present in the nucleus. The transient compartmentalization of Fyn in the nucleus could be important in nuclear signaling.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping