PUBLICATION
Muscle regulatory factor gene: zebrafish (Danio rerio) myogenin cDNA
- Authors
- Chen, Y.-H., Lee, W.-C., Cheng, C.-H., and Tsai, H.-J.
- ID
- ZDB-PUB-000927-1
- Date
- 2000
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 127(1): 97-103 (Journal)
- Registered Authors
- Chen, Yau-Hung, Lee, Wen-Chih, Tsai, Huai-Jen
- Keywords
- fish; helix-loop-helix; molecular structure; muscle-specific; myogenin; phylogenic tree; RACE; RT-PCR
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Cloning, Molecular
- DNA Primers/metabolism
- DNA, Complementary/metabolism
- Embryo, Nonmammalian
- Models, Genetic
- Molecular Sequence Data
- Myogenin/chemistry*
- Myogenin/genetics*
- Open Reading Frames
- Phylogeny
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Homology, Amino Acid
- Zebrafish/genetics*
- Zebrafish/metabolism*
- PubMed
- 11126756 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Chen, Y.-H., Lee, W.-C., Cheng, C.-H., and Tsai, H.-J. (2000) Muscle regulatory factor gene: zebrafish (Danio rerio) myogenin cDNA. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 127(1):97-103.
Abstract
Myogenin is one of the basic helix-loop-helix proteins that regulate muscle-specific gene expression. Using reverse transciption-polymerase chain reaction (RT-PCR), 5'- and 3'-rapid amplification of cDNA ends (RACE), zebrafish myogenin cDNA was cloned from mRNA of embryos at 10-96 h post-fertilization. The cDNA, at 1384 base pairs (bp), contained a 771-bp open reading frame with 113- and 500-bp flanking regions at the 5'- and 3'-ends, respectively. The deduced amino acid sequences of zebrafish myogenin encoded a 256-amino-acid polypeptide. In a comparison with myogenin of carp, trout, Xenopus, chicken and human, zebrafish myogenin shared 90.9, 77.6, 70.3, 62.9 and 51.5% amino acid identity, respectively. The basic helix-loop-helix domains in myogenin are all conserved. The molecular phylogenic tree demonstrated that myogenin of zebrafish is more closely related to that of fish than to the myogenin of other vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping