ZFIN ID: ZDB-PUB-000726-4
Molecular cloning and functional characterization of the zebrafish ATP-gated ionotropic receptor P2X3 subunit
Egan, T.M., Cox, J.A., and Voigt, M.M.
Date: 2000
Source: FEBS letters   475(3): 287-290 (Journal)
Registered Authors: Voigt, Mark
Keywords: transmitter-gated channel; purinoceptor; nucleotide; zebrafish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Molecular Sequence Data
  • Rats
  • Receptors, Purinergic P2/genetics*
  • Receptors, Purinergic P2/metabolism
  • Sequence Alignment
  • Zebrafish*
PubMed: 10869573 Full text @ FEBS Lett.
We describe a P2X subunit cloned from the zebrafish (Danio rerio) that is an orthologue of the mammalian P2X(3) subunit. Like the mammalian P2X(3), this receptor desensitizes rapidly in the presence of agonist. However, it differs in that alphabeta-meATP is a much less potent agonist than ATP and the antagonist TNP-ATP is not active at low nanomolar concentrations. Similar to the rat P2X(3) subunit, the zebrafish subunit forms hetero-oligomeric assemblies with the rat P2X(2) that possesses a phenotype distinct from either parent. This novel clone will provide an important basis for future experiments investigating the structure/function relationships of P2X subunit domains.