ZFIN ID: ZDB-PUB-000616-1
Novel mix-family homeobox genes in zebrafish and their differential regulation
Hirata, T., Yamanaka, Y., Ryu, S.L., Shimizu, T., Yabe, T., Hibi, M., and Hirano, T.
Date: 2000
Source: Biochemical and Biophysical Research Communications   271(3): 603-609 (Journal)
Registered Authors: Hibi, Masahiko, Hirata, Tsutomu, Ryu, Seung-Lim, Shimizu, Takashi, Yabe, Taijirou, Yamanaka, Yojiro
Keywords: zebrafish; homeoprotein; mtx; mix; YSL; wnt
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Chromosome Mapping
  • Cloning, Molecular
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental/genetics*
  • Homeodomain Proteins/chemistry
  • Homeodomain Proteins/genetics*
  • In Situ Hybridization
  • Microinjections
  • Molecular Sequence Data
  • Nodal Protein
  • Proto-Oncogene Proteins/genetics
  • RNA, Messenger/metabolism
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Transforming Growth Factor beta/genetics
  • Transforming Growth Factor beta/metabolism
  • Wnt Proteins
  • Zebrafish/embryology*
  • Zebrafish Proteins*
PubMed: 10814508 Full text @ Biochem. Biophys. Res. Commun.
We report the isolation of two novel zebrafish mix-type homeobox genes, mtx1 and mtx2. The homeodomains of both Mtx1 and Mtx2 exhibited a 50% amino acid identity to other Mix-family protein homeodomains. mtx1 was expressed throughout the yolk syncytial layer (YSL), an extraembryonic structure in teleosts, from the late-blastula to the mid-gastrula period. mtx2 was first expressed in the dorsal blastomeres soon after the mid-blastula transition, and slightly later in the entire blastoderm margin. After the late blastula period, mtx2 transcripts were detected in the YSL, and they were restricted to the dorsal YSL by the early gastrula period. The expression of mtx2 was dependent on Wnt signals but not on Nodal signals. mtx1 expression was not regulated by either Wnt or Nodal signals. This is in complete contrast to the Nodal signal-dependent expression of mixer. These results indicate the complexity of the regulation of mix-type homeobox genes.