PUBLICATION

mRNA localization patterns in zebrafish oocytes

Authors
Howley, C. and Ho, R.K.
ID
ZDB-PUB-000504-5
Date
2000
Source
Mechanisms of Development   92(2): 305-309 (Journal)
Registered Authors
Ho, Robert K., Howley, Cristin
Keywords
zebrafish; oogenesis; maternal mRNA localization; oocytes; eggs
MeSH Terms
  • Animals
  • Cyclin B/genetics
  • Cytoskeletal Proteins/genetics
  • Female
  • Gene Expression Regulation
  • Octamer Transcription Factor-3
  • Oocytes/physiology*
  • Oogenesis
  • Ovum/physiology
  • Proteins/genetics
  • RNA, Messenger/analysis
  • RNA, Messenger/metabolism*
  • RNA-Binding Proteins*
  • Trans-Activators*
  • Transcription Factors/genetics
  • Zebrafish
  • Zebrafish Proteins*
  • beta Catenin
PubMed
10727871 Full text @ Mech. Dev.
Abstract
In both invertebrate and vertebrate systems, the localization of maternal mRNAs is a common mechanism used to influence developmental processes, including the establishment of the dorsal/ventral axis, anterior/posterior axis, and the germ line (for review, see Bashirullah et al., 1998. Annu. Rev. Biochem. 67, 335-394). While the existence of localized maternal mRNAs has been reported in the zebrafish, Danio rerio, the precise localization patterns of these molecules during oogenesis has not been determined. In this study, in situ hybridization experiments were performed on zebrafish ovaries and activated eggs to examine different mRNA localization patterns. The results establish that while some maternal mRNAs remain ubiquitously distributed throughout the oocyte, other mRNAs follow specific localization patterns, including localization to the animal pole, localization to the vegetal pole, and cortical localization. The animal/vegetal axis is first apparent in stage II oocytes when the earliest mRNA localization is seen. Unique patterns of localization are seen in mature eggs as well. Some mRNAs maintain their oocyte localization patterns, while others localize upon egg activation (fertilization).
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Human Disease / Model
Sequence Targeting Reagents
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