PUBLICATION
Reverse genetics in zebrafish
- Authors
- Lekven, A.C., Helde, K.A., Thorpe, C.J., Rooke, R. and Moon, R.T.
- ID
- ZDB-PUB-000330-2
- Date
- 2000
- Source
- Physiological Genomics 2: 37-48 (Journal)
- Registered Authors
- Helde, Kathryn, Lekven, Arne, Moon, Randall T., Rooke, Rebecca, Thorpe, Chris
- Keywords
- mutagenesis; trimethylpsoralen; gamma rays; ethylnitrosourea; functional genomics
- MeSH Terms
-
- Animals
- Blastocyst/radiation effects
- Chromosome Deletion
- DNA Mutational Analysis/methods
- Gene Targeting
- Genetic Complementation Test
- Genetic Techniques*
- Genetic Testing/methods
- Heteroduplex Analysis
- Models, Biological
- Mutagenesis, Site-Directed
- Polymerase Chain Reaction
- Sequence Deletion
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 11015580 Full text @ Physiol. Genomics
Citation
Lekven, A.C., Helde, K.A., Thorpe, C.J., Rooke, R. and Moon, R.T. (2000) Reverse genetics in zebrafish. Physiological Genomics. 2:37-48.
Abstract
The zebrafish has become a popular model system for the study of vertebrate developmental biology because of its numerous strengths as a molecular genetic and embryological system. To determine the requirement for specific genes during embryogenesis, it is necessary to generate organisms carrying loss-of-function mutations. This can be accomplished in zebrafish through a reverse genetic approach. This review discusses the current techniques for generating mutations in known genes in zebrafish. These techniques include the generation of chromosomal deletions and the subsequent identification of complementation groups within deletions through noncomplementation assays. In addition, this review will discuss methods currently being evaluated that may improve the methods for finding mutations in a known sequence, including screening for randomly induced small deletions within genes and screening for randomly induced point mutations within specific genes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping