ZFIN ID: ZDB-PUB-000327-1
Characterization of a zebrafish/mouse somatic cell hybrid panel
Chevrette, M., Joly, L., Tellis, P., Knapik, E.W., Miles, J., Fishman, M., and Ekker, M.
Date: 2000
Source: Genomics   64(1): 119-26 (Journal)
Registered Authors: Chevrette, Mario, Ekker, Marc, Fishman, Mark C., Joly, Lucille, Knapik, Ela W., Miles, Jennifer, Tellis, Patricia
Keywords: none
MeSH Terms:
  • Animals
  • Cell Line
  • Genetic Linkage
  • Genetic Markers
  • Hybrid Cells
  • In Situ Hybridization, Fluorescence
  • Mice
  • Microsatellite Repeats
  • Polymerase Chain Reaction
  • Zebrafish/genetics*
PubMed: 10708527 Full text @ Genomics
We have characterized a collection of zebrafish/mouse somatic cell hybrids with 211 genes and markers chosen from the 25 zebrafish linkage groups. Most of the zebrafish genome is represented in this collection with 88% of genes/markers present in at least one hybrid cell line. Although most hybrids contain chromosomal fragments, there are a few instances where a complete or nearly complete zebrafish chromosome has been maintained in a mouse background, based on multiple markers covering the entire chromosome. In addition to their use in mapping studies, this collection of somatic cell hybrids should constitute an important tool as a source of specific chromosome fragments and for assessing the function of genome regions.