Effects of dopamine depletion on visual sensitivity of zebrafish
- Li, L. and Dowling, J.E.
- The Journal of neuroscience : the official journal of the Society for Neuroscience 20(5): 1893-903 (Journal)
- Registered Authors
- Dowling, John E., Li, Lei
- 6-OHDA; circadian clock; dark adaptation; dopamine; dopamine receptor agonist; ADTN; ERG recording; RGC recording; visual sensitivity; zebrafish
- MeSH Terms
- Circadian Rhythm/physiology
- Color Perception/physiology*
- Dark Adaptation/physiology
- Dopamine Agonists/pharmacology
- Microscopy, Confocal
- Photic Stimulation
- Receptors, Dopamine/physiology
- Retinal Cone Photoreceptor Cells/chemistry
- Retinal Cone Photoreceptor Cells/metabolism
- Retinal Degeneration/chemically induced
- Retinal Degeneration/genetics
- Retinal Degeneration/physiopathology
- Retinal Rod Photoreceptor Cells/chemistry
- Retinal Rod Photoreceptor Cells/metabolism
- Sensory Thresholds/physiology*
- 10684890 Full text @ J. Neurosci.
Li, L. and Dowling, J.E. (2000) Effects of dopamine depletion on visual sensitivity of zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 20(5):1893-903.
The visual sensitivity of zebrafish in which the retinal dopaminergic interplexiform cells (DA-IPCs) were destroyed by 6-hydroxydopamine was measured behaviorally. During the first 6-8 min of dark adaptation, visual thresholds of DA-IPC-depleted animals were similar to those of control animals. Thereafter, their visual thresholds were elevated so that by 14-18 min of dark adaptation, they were 2-3 log units above those of control animals. In DA-IPC-depleted animals, the electroretinogram was normal in terms of light sensitivity and waveform, but the light threshold for eliciting a ganglion cell discharge was raised by 1.8 log units as compared with control animals. No obvious rod system function was detected in DA-IPC-depleted animals as measured behaviorally. Partial rescue of the behavioral visual sensitivity loss in DA-IPC-depleted animals occurred when dopamine or a long-acting dopamine agonist (2-amino-6, 7-dihydroxy-1, 2, 3, 4-tetrahydronaphthalene hydrobromide) were injected intraocularly. Our data suggest that the principal visual defect shown by DA-IPC-depleted animals is attributable to effects occurring in the inner retina, mainly on rod signals. We also show that dopamine is involved in mediating the effect of the circadian clock on visual sensitivity.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes