PUBLICATION
Growth cone and dendrite dynamics in zebrafish embryos: early events in synaptogenesis imaged in vivo
- Authors
- Jontes, J.D., Buchanan, J., and Smith, S.J.
- ID
- ZDB-PUB-000309-29
- Date
- 2000
- Source
- Nature Neuroscience 3(3): 231-237 (Journal)
- Registered Authors
- Jontes, James
- Keywords
- none
- MeSH Terms
-
- Animals
- Axons/metabolism
- Axons/ultrastructure
- Cell Movement
- Cell Size
- Dendrites/metabolism*
- Embryo, Nonmammalian/cytology
- Fluorescent Dyes
- Growth Cones/metabolism*
- Microscopy, Electron
- Microscopy, Fluorescence
- Motor Neurons/cytology*
- Motor Neurons/metabolism
- Motor Neurons/ultrastructure
- Pseudopodia/metabolism
- Spinal Cord/cytology*
- Spinal Cord/embryology
- Synapses/metabolism*
- Synapses/ultrastructure
- Synaptic Vesicles/ultrastructure
- Time Factors
- Zebrafish/embryology
- PubMed
- 10700254 Full text @ Nat. Neurosci.
Citation
Jontes, J.D., Buchanan, J., and Smith, S.J. (2000) Growth cone and dendrite dynamics in zebrafish embryos: early events in synaptogenesis imaged in vivo. Nature Neuroscience. 3(3):231-237.
Abstract
We used time-lapse fluorescence microscopy to observe the growth of Mauthner cell axons and their postsynaptic targets, the primary motor neurons, in spinal cords of developing zebrafish embryos. Upon reaching successive motor neurons, the Mauthner growth cone paused briefly before continuing along its path. Varicosities formed at regular intervals and were preferentially associated with the target regions of the primary motor neurons. In addition, the postsynaptic motor neurons showed highly dynamic filopodia, which transiently interacted with both the growth cone and the axon. Both Mauthner cell and motor neurons were highly active, each showing motility sufficient to initiate synaptogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping