PUBLICATION
Nucleotide sequence of a cDNA clone coding for an intestinal-type fatty acid binding protein and its tissue-specific expression in zebrafish (Danio rerio)
- Authors
- Pierce, M., Wang, Y.M., Denovan-Wright, E.M., and Wright, J.M.
- ID
- ZDB-PUB-000301-17
- Date
- 2000
- Source
- Biochim. Biophys. Acta Gene Struct. Exp. 1490(1-2): 175-183 (Journal)
- Registered Authors
- Wright, Jonathan M.
- Keywords
- IFABP; cDNA cloning; in situ hybridization; Northern analysis
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Autoradiography
- Base Sequence
- Carrier Proteins/genetics*
- Carrier Proteins/metabolism
- Cloning, Molecular
- Fatty Acid-Binding Proteins
- Female
- Gene Expression Regulation
- In Situ Hybridization
- Intestines/metabolism
- Male
- Microvilli/metabolism
- Molecular Sequence Data
- Myelin P2 Protein/genetics*
- Myelin P2 Protein/metabolism
- Neoplasm Proteins*
- Nerve Tissue Proteins*
- RNA, Messenger/analysis
- Sequence Alignment
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins*
- PubMed
- 10786634 Full text @ Biochim. Biophys. Acta Gene Struct. Exp.
Citation
Pierce, M., Wang, Y.M., Denovan-Wright, E.M., and Wright, J.M. (2000) Nucleotide sequence of a cDNA clone coding for an intestinal-type fatty acid binding protein and its tissue-specific expression in zebrafish (Danio rerio). Biochim. Biophys. Acta Gene Struct. Exp.. 1490(1-2):175-183.
Abstract
We have cloned a cDNA from zebrafish (Danio rerio) that contains an open-reading frame of 132 amino acids coding for a fatty acid binding protein (FABP) of approximately 15 kDa. Multiple sequence alignment revealed extensive amino acid identity between this zebrafish FABP and intestinal-like FABPs (I-FABP) from other species. The zebrafish I-FABP cDNA hybridized to single restriction fragments of total zebrafish genomic DNA digested with the restriction endonucleases PstI Bg/II or EcoRI suggesting that a single copy of the I-FABP gene is present in the zebrafish genome. An oligonucleotide probe complementary to the zebrafish I-FABP mRNA hybridized to an mRNA of approximately 800 bases in Northern blot analysis. In situ hybridization revealed that the I-FABP mRNA was expressed exclusively in the intestine of the adult zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping