ZFIN ID: ZDB-PERS-980520-4
Chan, Joanne
Affiliation: Joanne Chan Lab
Address: Vascular Biology Program, Children's Hospital New Research Building, 12th floor, Room 12.217 300 Longwood Avenue Boston, MA 02115-5737 USA
Country: United States
Phone: (617) 919-2379
Fax: (617) 730-0231

I have always been interested in receptor tyrosine kinase signaling pathways in normal physiology and in disease conditions. The zebrafish provides an ideal model system to examine signal transduction in an intact vertebrate organism. My research is focused on blood vessel formation as it relates to tumor angiogenesis during cancer progression.

Like many zebrafish researchers, I am still amazed by the optical clarity of the transparent zebrafish embryo and its rapid development over the first few days of life. It is especially well-suited for vascular biology studies, as blood flow and blood vessel function can be easily visualized using a dissecting microscope. As a vertebrate organism, its genes are highly conserved with human genes, so that data generated from the zebrafish system can be applicable to humans. New technologies are constantly being developed by the zebrafish community. These combined advantages contribute to the current enthusiasm over the use of the fish as a model system.

Our laboratory is focused on critical genes that control endothelial cell function and blood vessel formation. We are pursuing a sensitized genetic screen for mutations in angiogenic genes. It is our hope that positional cloning of these mutants will reveal critical genes for angiogenesis that might become novel targets for anti-angiogenic therapies.

Kawasaki, J., Aegerter, S., Fevurly, R.D., Mammoto, A., Mammoto, T., Sahin, M., Mably, J.D., Fishman, S.J., Chan, J. (2014) RASA1 functions in EPHB4 signaling pathway to suppress endothelial mTORC1 activity. J. Clin. Invest.. 124(6):2774-84
Jia, D., Hasso, S.M., Chan, J., Filingeri, D., D'Amore, P.A., Rice, L., Pampo, C., Siemann, D.W., Zurakowski, D., Rodig, S.J., and Moses, M.A. (2013) Transcriptional repression of VEGF by ZNF24: mechanistic studies and vascular consequences in vivo. Blood. 121(4):707-715
Fevurly, R.D., Hasso, S., Fye, A., Fishman, S.J., and Chan, J. (2012) Novel zebrafish model reveals a critical role for MAPK in lymphangiogenesis. Journal of Pediatric Surgery. 47(1):177-182
Hasso, S., and Chan, J. (2011) Chemical approaches to angiogenesis in development and regeneration. Methods in cell biology. 101:181-195
Chan, J., and Mably, J.D. (2011) Dissection of cardiovascular development and disease pathways in zebrafish. Progress in molecular biology and translational science. 100:111-153
Bolcome, R.E. 3rd, and Chan, J. (2010) Constitutive MEK1 Activation Rescues Anthrax Lethal Toxin-Induced Vascular Effects In Vivo. Infection and Immunity. 78(12):5043-5053
Vasil, M.L., Stonehouse, M.J., Vasil, A.I., Wadsworth, S.J., Goldfine, H., Bolcome, R.E. 3rd, and Chan, J. (2009) A complex extracellular sphingomyelinase of Pseudomonas aeruginosa inhibits angiogenesis by selective cytotoxicity to endothelial cells. PLoS pathogens. 5(5):e1000420
Bolcome, R.E. 3rd, Sullivan, S.E., Zeller, R., Barker, A.P., Collier, R.J., and Chan, J. (2008) Anthrax lethal toxin induces cell death-independent permeability in zebrafish vasculature. Proceedings of the National Academy of Sciences of the United States of America. 105(7):2439-2444
Juris, S.J., Melnyk, R.A., Bolcome, R.E., Chan, J., and Collier, R.J (2007) Crosslinked Forms of the Isolated N-terminal Domain of the Lethal Factor are Potent Inhibitors of Anthrax Toxin. Infection and Immunity. 75(10):5052-5058
Ho, D.M., Chan, J., Bayliss, P., and Whitman, M. (2006) Inhibitor-resistant type I receptors reveal specific requirements for TGF-beta signaling in vivo. Developmental Biology. 295(2):730-742
Wagle, M., Grunewald, B., Subburaju, S., Barzaghi, C., Le Guyader, S., Chan, J., and Jesuthasan, S. (2004) EphrinB2a in the zebrafish retinotectal system. Journal of neurobiology. 59(1):57-65
Chan, J., and Serluca, F.C. (2004) Chemical approaches to angiogenesis. The Zebrafish: Cellular and Developmental Biology,2nd Ed. Methods Cell Biol.. 76:475-487
Montero, J.-A., Killan, B., Chan, J., Bayliss, P.E., and Heisenberg, C.-P. (2003) Phosphoinositide 3-kinase is required for process outgrowth and cell polarization of gastrulating mesendodermal cells. Current biology : CB. 13(15):1279-1289
Chan, J., Bayliss, P.E., Wood, J.M., and Roberts, T.M. (2002) Dissection of angiogenic signaling in zebrafish using a chemical genetic approach. Cancer Cell. 1(3):257-267
Chan, J., Mably, J.D., Serluca, F.C., Chen, J.-N., Goldstein, N.B., Thomas, M.C., Cleary, J.A., Brennan, C., Fishman, M.C., and Roberts, T.M. (2001) Morphogenesis of prechordal plate and notochord requires intact Eph/Ephrin B signaling. Developmental Biology. 234(2):470-482