ZFIN ID: ZDB-PERS-960805-219
Hackett, Perry B.
Email: perry@cbs.umn.edu
URL: http://biosci.cbs.umn.edu/labs/perry/
Affiliation: Hackett Lab
Address: Department of Genetics, Cell Biology and Development University of Minnesota 6-106 Jackson Hall 321 Church Street SE and Discovery Genomics, Inc., 614 McKinley Place, NE, 55413 Minneapolis, MN 55455 USA
Country: United States
Phone: (612) 624-6736
Fax: (612) 625-5754


Web: http://www.discoverygenomics.net
Email: perryh@discoverygenomics.net
Phone: (612) 656-4484
Fax: (612) 379-6580


Developing methods for gene delivery to cells for three purposes: (1) conferring new traits, (2) tagging genes for determining their functions, and (3) site-specific recombination to inactivate expression of specific genes. For the first two goals we made a transposon vector (Sleeping Beauty) that is useful for insertional mutagenesis, gene tagging, and enhancer/gene/poly(A) trapping as well as transgenesis. The current lab efforts are directed to enhancing the activities of the SB Transposon system for gene delivery to mammals and fish as well as for insertional mutagenesis/gene-tagging.

Song, G., Li, Q., Long, Y., Gu, Q., Hackett, P.B., and Cui, Z. (2012) Effective gene trapping mediated by sleeping beauty transposon. PLoS One. 7(8):e44123
Wang, Y., Kaiser, M.S., Larson, J.D., Nasevicius, A., Clark, K.J., Wadman, S.A., Roberg-Perez, S.E., Ekker, S.C., Hackett, P.B., McGrail, M., and Essner, J.J. (2010) Moesin1 and Ve-cadherin are required in endothelial cells during in vivo tubulogenesis. Development (Cambridge, England). 137(18):3119-3128
Kalén, M., Wallgard, E., Asker, N., Nasevicius, A., Athley, E., Billgren, E., Larson, J.D., Wadman, S.A., Norseng, E., Clark, K.J., He, L., Karlsson-Lindahl, L., Häger, A.K., Weber, H., Augustin, H., Samuelsson, T., Kemmet, C.K., Utesch, C.M., Essner, J.J., Hackett, P.B., and Hellström, M. (2009) Combination of reverse and chemical genetic screens reveals angiogenesis inhibitors and targets. Chemistry & Biology. 16(4):432-441
Balciunas, D., Wangensteen, K.J., Wilber, A., Bell, J., Geurts, A., Sivasubbu, S., Wang, X., Hackett, P.B., Largaespada, D.A., McIvor, R.S., and Ekker, S.C. (2006) Harnessing a High Cargo-Capacity Transposon for Genetic Applications in Vertebrates. PLoS Genetics. 2(11):e169
Larson, J.D., Wadman, S.A., Chen, E., Kerley, L., Clark, K.J., Eide, M., Lippert, S., Nasevicius, A., Ekker, S.C., Hackett, P.B., and Essner, J.J. (2004) Expression of VE-cadherin in zebrafish embryos: A new tool to evaluate vascular development. Developmental dynamics : an official publication of the American Association of Anatomists. 231(1):204-213
Clark, K.J., Geurts, A.M., Bell, J.B., and Hackett, P.B. (2004) Transposon vectors for gene-trap insertional mutagenesis in vertebrates. Genesis (New York, N.Y. : 2000). 39(4):225-233
Davidson, A.E., Balciunas, D., Mohn, D., Shaffer, J., Hermanson, S., Sivasubbu, S., Cliff, M.P., Hackett, P.B., and Ekker, S.C. (2003) Efficient gene delivery and gene expression in zebrafish using the Sleeping Beauty transposon. Developmental Biology. 263(2):191-202
Cui, Z., Yang, Y., Kaufman, C.D., Agalliu, D., and Hackett, P.B. (2003) RecA-mediated, targeted mutagenesis in zebrafish. Marine biotechnology (New York, N.Y.). 5(2):174-184
Dupuy, A.J., Clark, K., Carlson, C.M., Fritz, S., Davidson, A.E., Markley, K.M., Finley, K., Fletcher, C.F., Ekker, S.C., Hackett, P.B., Horn, S., and Largaespada, D.A. (2002) Mammalian germ-line transgenesis by transposition. Proceedings of the National Academy of Sciences of the United States of America. 99(7):4495-4499
Cui, Z., Clark, K.J., Kaufman, C.D., and Hackett, P.B. (2001) Inhibition of skiA and skiB gene expression ventralizes zebrafish embryos. Genesis (New York, N.Y. : 2000). 30(3):149-153
Fahrenkrug, S.C., Joshi, B., Hackett, P.B., Jr., and Jagus, R. (2000) Alternative transcriptional initiation and splicing define the translational efficiencies of zebrafish mRNAs encoding eukaryotic initiation factor 4E. Differentiation; research in biological diversity. 66(1):15-22
Kaufman, C.D., Martinez-Rodriguez, G., and Hackett, P.B. (2000) Ectopic expression of c-ski disrupts gastrulation and neural patterning in zebrafish. Mechanisms of Development. 95(1-2):147-162
Hackett, P.B. and Alvarez, M.C. (2000) The molecular genetics of transgenic fish. Rec. Adv. Mar. Biotech.. 4:77-145
Fahrenkrug, S.C., Dahlquist, M.O., Clark, K.J., and Hackett, P.B. (1999) Dynamic and tissue-specific expression of elF4E during zebrafish embryogenesis. Differentiation; research in biological diversity. 65(4):191-201
Fahrenkrug, S.C., Clark, K., Dahlquist, M.O., and Hackett, P.B. (1999) Dicistronic gene expression in developing zebrafish. Marine biotechnology (New York, N.Y.). 1(6):552-561
Caldovic, L., Agalliu, D., and Hackett, P.B. (1999) Position-independent expression of transgenes in zebrafish. Transgenic Research. 8(5):321-334
Essner, J.J., Johnson, R.G., and Hackett, P.B., Jr. (1999) Overexpression of thyroid hormone receptor alpha1 during zebrafish embryogenesis disrupts hindbrain patterning and implicates retinoic acid receptors in the control of hox gene expression. Differentiation; research in biological diversity. 65(1):1-11
Hackett, P.B., Izsvák, Z., Ivics, Z., and Caldovic, L. (1999) Development of genetic tools for transgenic animals. In Transgenic Animals in Agriculture. CAB International, Wllingford, UK. :19-35
Ivics, Z., Izsvák, and Hackett, P.B. (1999) Genetic applications of transposons and other repetitive elements in zebrafish. In The Zebrafish: Genetics and Genomics, H.W. Detrich, III, M. Westerfield, and L.I. Zon, eds., San Diego, CA: Academic Press, Methods Cell Biol.. 60:99-131
Izsvák, Z., Ivics, Z., Shimoda, N., Mohn, D., Okamoto, H., and Hackett, P.B. (1999) Short inverted-repeat transposable elements in teleost fish and implications for a mechanism of their amplification. Journal of molecular evolution. 48:13-21
Essner, J.J., Breuer, J.J., Essner, R.D., Fahrenkrug, S.C., and Hackett, P.B., Jr. (1997) The zebrafish thyroid hormone receptor alpha1 is expressed during early embryogenesis and can function in transcriptional repression. Differentiation; research in biological diversity. 62:107-117
Izsvák, Z., Ivics, Z., Hackett, P.B. (1997) Repetitive elements and their genetic applications in zebrafish. Biochemistry and cell biology = Biochimie et biologie cellulaire. 75:507-523
Essner, J.J., Laing, J.G., Beyer, E.C., Johnson, R.G., and Hackett, P.B. Jr. (1996) Expression of zebrafish connexin43.4 in the notochord and tail bud of wild-type and mutant no tail embryos. Developmental Biology. 177(2):449-462
Izsvák, Z., Ivics, Z., Garcia-Estefania, D., Fahrenkrug, S.C., and Hackett, P.B. (1996) DANA elements: A family of composite, tRNA-derived short interspersed DNA elements associated with mutational activities in zebrafish (Danio rerio). Proceedings of the National Academy of Sciences of the United States of America. 93(3):1077-1081
Hackett, P.B. (1996) Genetic engineering of Minnesota superfish. SAAS bulletin, biochemistry and biotechnology. 9:69-76
Izsvák, Z., Ivics, Z., Hackett, P.B. (1995) Characterization of a Tc1-like transposable element in zebrafish (Danio rerio). Molecular & general genetics : MGG. 247:312-322
Caldovic, L., and Hackett, P.B. Jr. (1995) Development of position-independent expression vectors and their transfer into transgenic fish. Molecular marine biology and biotechnology. 4:51-61
Ivics, Z., Izsvák, Z., Hackett, P.B. (1993) Enhanced incorporation of transgenic DNA into zebrafish chromosomes by a retroviral integration protein. Molecular marine biology and biotechnology. 2:162-173
Moav, B., Liu, Z., Caldovic, L.D., Gross, M.L., Faras, A.J., and Hackett, P.B. (1993) Regulation of expression of transgenes in developing fish. Transgenic Research. 2:153-161
He, L., Zhu, Z., Faras, A.J., Guise, K.S., Hackett, P.B., and Kapuscinski, A.R. (1992) Characterization of AluI repeats of zebrafish (Brachydanio rerio). Molecular marine biology and biotechnology. 1:125-135

Hackett, P.B., K.J. Clark, S.E. Ekker and J.J. Essner (2004). Applications of Transposable Elements in Fish for Transgenesis and Functional Genomics. Fish Development and Genetics (Z. Gong and V. Korzh, eds.) Chapter 16, 532-580.

Liu, G., A.M. Geurts, K. Yae, A.R. Srinivassan, S.C. Fahrenkrug, W.K. Olson J. Takeda, K. Horie and P.B. Hackett (2005). Target-site preference for Sleeping Beauty transposons. J. Mol. Biol. 346: 161-173.
[This paper describes the "randomness of integration - an important feature for using the SB transposon system for both gene delivery to fish chromosomes and insertional mutagenesis.]

Wadman, S.A., K.J. Clark and P.B. Hackett (2005). Fishing for answers with transposons. Mar. Biotech. 7: (in press).