ZFIN ID: ZDB-PERS-121026-2
Legradi, Jessica
Email: Jessica.Legradi@vu.nl
URL: https://www.researchgate.net/profile/Jessica_Legradi2
Affiliation: Legradi Lab
Address: VU Amsterdam, Faculty of Science, Department of Environment and Health De Boelelaan 1087, 1081 HV Amsterdam, Netherlands e-mail: Jessica.Legradi@vu.nl
Country: Netherlands
Phone:
Fax:
ORCID ID: 0000-0003-0388-860X


BIOGRAPHY AND RESEARCH INTERESTS
I work since 2017 as an assistant Professor at the department of Environment and Healthy at the VU Amsterdam. I teache toxicology at BSc and MSc level. My research focuses on studying the toxicological effects of environmental contaminants using zebrafish (Danio rerio). I identify toxic mechanism of action of environmental pollutants and develop new specific bioassays also applicable for chemical toxicity screening. Within several European projects I characterized the toxic potential and identified the mechanisms of toxicity of different chemicals found in the environment. Therefore I applied and adapted state of the art genomics, proteomics and metabolomics technologies. Moreover, I worked on biological test systems that can guide contaminant detection in complex environmental samples by combining bioassays with analytical chemical analysis methods (EDA). During the last years I developed a strong interest for behavioural testing and neurotoxicity.

My Research Interests:
Mechanisms of toxicity, developmental toxicology, ecotoxicology, effect directed analysis, neurotoxicology, behavioural studies

https://www.researchgate.net/profile/Jessica_Legradi2


PUBLICATIONS
Chelcea, I., Vogs, C., Hamers, T., Koekkoek, J., Legradi, J., Sapounidou, M., Örn, S., Andersson, P.L. (2023) Physiology-informed toxicokinetic model for the zebrafish embryo test developed for bisphenols. Chemosphere. 345:140399
Xu, M., Legradi, J., Leonards, P. (2023) A comprehensive untargeted metabolomics study in zebrafish embryos exposed to perfluorohexane sulfonate (PFHxS). The Science of the total environment. 887:163770
Gustafsson, J., Legradi, J., Lamoree, M.H., Asplund, L., Leonards, P.E.G. (2022) Metabolite alterations in zebrafish embryos exposed to hydroxylated polybrominated diphenyl ethers. The Science of the total environment. 857(Pt 1):159269
Chelcea, I., Örn, S., Hamers, T., Koekkoek, J., Legradi, J., Vogs, C., Andersson, P.L. (2022) Physiologically Based Toxicokinetic Modeling of Bisphenols in Zebrafish (Danio rerio) Accounting for Variations in Metabolic Rates, Brain Distribution, and Liver Accumulation. Environmental science & technology. 56(14):10216-10228
Thessen, A.E., Marvel, S., Achenbach, J.C., Fischer, S., Haendel, M.A., Hayward, K., Klüver, N., Könemann, S., Legradi, J., Lein, P., Leong, C., Mylroie, J.E., Padilla, S., Perone, D., Planchart, A., Prieto, R.M., Muriana, A., Quevedo, C., Reif, D., Ryan, K., Stinckens, E., Truong, L., Vergauwen, L., Vom Berg, C., Wilbanks, M., Yaghoobi, B., Hamm, J. (2022) Implementation of Zebrafish Ontologies for Toxicology Screening. Frontiers in toxicology. 4:817999
Haigis, A.C., Ottermanns, R., Schiwy, A., Hollert, H., Legradi, J. (2022) Getting more out of the zebrafish light dark transition test. Chemosphere. 295:133863
Xu, M., Legradi, J., Leonards, P. (2021) Using comprehensive lipid profiling to study effects of PFHxS during different stages of early zebrafish development. The Science of the total environment. 808:151739
Xu, M., Legradi, J., Leonards, P. (2021) Cross platform solutions to improve the zebrafish polar metabolome coverage using LC-QTOF MS: Optimization of separation mechanisms, solvent additives, and resuspension solvents. Talanta. 234:122688
Pípal, M., Legradi, J., Smutná, M., Kočí, T., Priebojová, J., Bláhová, L., Krauss, M., Hilscherová, K. (2020) Neurobehavioral effects of cyanobacterial biomass field extracts on zebrafish embryos and potential role of retinoids. Aquatic toxicology (Amsterdam, Netherlands). 228:105613
Xu, M., Legradi, J., Leonards, P. (2020) Evaluation of LC-MS and LC×LC-MS in analysis of zebrafish embryo samples for comprehensive lipid profiling. Analytical and bioanalytical chemistry. 412(18):4313-4325
Spaan, K., Haigis, A.C., Weiss, J., Legradi, J. (2019) Effects of 25 thyroid hormone disruptors on zebrafish embryos: A literature review of potential biomarkers. The Science of the total environment. 656:1238-1249
Chen, Q., Yin, D., Jia, Y., Schiwy, S., Legradi, J., Yang, S., Hollert, H. (2017) Enhanced uptake of BPA in the presence of nanoplastics can lead to neurotoxic effects in adult zebrafish. The Science of the total environment. 609:1312-1321
Björnsdotter, M.K., Jonker, W., Legradi, J., Kool, J., Ballesteros-Gómez, A. (2017) Bisphenol A alternatives in thermal paper from the Netherlands, Spain, Sweden and Norway. Screening and potential toxicity. The Science of the total environment. 601-602:210-221
Kopp, R., Martínez, I.O., Legradi, J., Legler, J. (2017) Exposure to endocrine disrupting chemicals perturbs lipid metabolism and circadian rhythms. Journal of environmental sciences (China). 62:133-137
Legradi, J., Pomeren, M.V., Dahlberg, A.K., Legler, J. (2017) Effects of Hydroxylated Polybrominated Diphenyl Ethers in Developing Zebrafish Are Indicative of Disruption of Oxidative Phosphorylation. International Journal of Molecular Sciences. 18(5)
Hausen, J., Otte, J.C., Legradi, J., Yang, L., Strähle, U., Fenske, M., Hecker, M., Tang, S., Hammers-Wirtz, M., Hollert, H., Keiter, S.H., Ottermanns, R. (2017) Fishing for contaminants: identification of three mechanism specific transcriptome signatures using Danio rerio embryos. Environmental science and pollution research international. 25(5):4023-4036
Aragon, A., Legradi, J., Ballesteros-Gómez, A., Legler, J., van Velzen, M., de Boer, J., Leonards, P. (2017) Determination of monoamine neurotransmitters in zebrafish (Danio rerio) by gas chromatography coupled to mass spectrometry with a two-step derivatization. Analytical and bioanalytical chemistry. 409(11):2931-2939
Hilinski, W.C., Bostrom, J.R., England, S.J., Juárez-Morales, J.L., de Jager, S., Armant, O., Legradi, J., Strähle, U., Link, B.A., Lewis, K.E. (2016) Lmx1b is required for the glutamatergic fates of a subset of spinal cord neurons. Neural Development. 11:16
Kopp, R., Billecke, N., Legradi, J., den Broeder, M., Parekh, S.H., Legler, J. (2016) Bringing obesity to light: Rev-erbα, a central player in light-induced adipogenesis in the zebrafish?. International journal of obesity (2005). 40(5):824-32
Kopp, R., Legler, J., Legradi, J. (2016) Alterations in locomotor activity of feeding zebrafish larvae as a consequence of exposure to different environmental factors. Environmental science and pollution research international. 25(5):4085-4093
Tufi, S., Leonards, P.E., Lamoree, M.H., De Boer, J., Legler, J., Legradi, J. (2016) Changes in neurotransmitter profiles during early zebrafish (Danio rerio) development and after pesticide exposure. Environmental science & technology. 50(6):3222-30
Dahlberg, A.K., Bignert, A., Legradi, J., Legler, J., Asplund, L. (2016) Anthropogenic and naturally produced brominated substances in Baltic herring (Clupea harengus membras) from two sites in the Baltic Sea. Chemosphere. 144:2408-2414
Legradi, J., El Abdellaoui, N., van Pomeren, M., Legler, J. (2015) Comparability of behavioural assays using zebrafish larvae to assess neurotoxicity. Environmental science and pollution research international. 22(21):16277-89
Legradi, J., Dahlberg, A.K., Cenijn, P., Marsh, G., Asplund, L., Bergman, A., Legler, J. (2014) Disruption of Oxidative Phosphorylation (OXPHOS) by Hydroxylated Polybrominated Diphenyl Ethers (OH-PBDEs) Present in the Marine Environment. Environmental science & technology. 48(24):14703-11
Bluhm, K., Otte, J.C., Yang, L., Zinsmeister, C., Legradi, J., Keiter, S., Kosmehl, T., Braunbeck, T., Strähle, U., Hollert, H. (2014) Impacts of Different Exposure Scenarios on Transcript Abundances in Danio rerio Embryos when Investigating the Toxicological Burden of Riverine Sediments. PLoS One. 9:e106523
Armant, O., März, M., Schmidt, R., Ferg, M., Diotel, N., Ertzer, R., Bryne, J.C., Yang, L., Baader, I., Reischl, M., Legradi, J., Mikut, R., Stemple, D., Ijcken, W.V., van der Sloot, A., Lenhard, B., Strähle, U., and Rastegar, S. (2013) Genome-wide, whole mount in situ analysis of transcriptional regulators in zebrafish embryos. Developmental Biology. 380(2):351-62
Ho, N.Y., Yang, L., Legradi, J., Armant, O., Takamiya, M., Rastegar, S., and Strähle, U. (2013) Gene Responses in the Central Nervous System of Zebrafish Embryos Exposed to the Neurotoxicant Methyl Mercury. Environmental science & technology. 47(7):3316-25
Kosmehl, T., Otte, J.C., Yang, L., Legradi, J., Bluhm, K., Zinsmeister, C., Keiter, S.H., Reifferscheid, G., Manz, W., Braunbeck, T., Strähle, U., and Hollert, H. (2012) A combined DNA-microarray and mechanism-specific toxicity approach with zebrafish embryos to investigate the pollution of river sediments. Reproductive toxicology (Elmsford, N.Y.). 33(2):245-253
Alshut, R., Legradi, J., Liebel, U., Yang, L., van Wezel, J., Strähle, U., Mikut, R., and Reischl, M. (2010) Methods for Automated High-Throughput Toxicity Testing Using Zebrafish Embryos. KI 2010: Advances in Artificial Intelligence. 6359:219-226
Alshut, R., Legradi, J., Mikut, R., Strähle, U., and Reischl, M. (2009) Robust Identification of Coagulated Zebrafish Eggs using Image Processing and Classification Techniques. Proceedings 19 Workshop Computational Intelligence. 50:9-21
Yang, L., Ho, N.Y., Alshut, R., Legradi, J., Weiss, C., Reischl, M., Mikut, R., Liebel, U., Müller, F., and Strähle, U. (2009) Zebrafish embryos as models for embryotoxic and teratological effects of chemicals. Reproductive toxicology (Elmsford, N.Y.). 28(2):245-253
Yang, L., Kemadjou, J.R., Zinsmeister, C., Legradi, J., Bauer, M., Muller, F., Pankratz, M., Jakel, J., and Straehle, U. (2007) Transcriptional profiling reveals barcode-like toxicogenomic responses in the zebrafish embryo. Genome biology. 8(10):R227

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