ZFIN Person: Moro, Enrico

ZFIN ID: ZDB-PERS-060706-6

ZFIN ID: ZDB-PERS-060706-6

Moro, Enrico

Email:	moroe@bio.unipd.it, enrico.moro.1@unipd.it
URL:	https://en.didattica.unipd.it/off/docente/6AAF7178FAD13F45A60A6B6AB11772EA
Affiliation:	Moro Lab
Address:	Department of Molecular Medicine University of Padova Via U. Bassi 58/B Padova, 35131 Italy
Country:	Italy
Phone:	39-49-827-6341
Fax:	+390498276079
ORCID ID:	0000-0003-3166-8102

BIOGRAPHY AND RESEARCH INTERESTS

My group is currently involved in using zebrafish to model lysosomal storage disorders (LSD), particularly the Hunter syndrome and Gaucher disease. We are exploiting several in vivo approaches to dissect early molecular events involved in cellular abnormalities detected in fish morphants and mutants for lysosome-associated proteins. We are particularly focused in the bone and heart compartments, as in some of the investigated disorders these tissues are severely compromised.
We are also trying to translate the observed results in mouse models and perform also a preliminary molecular characterization of affected patients.

PUBLICATIONS

Ferlazzo, G.M., Gambetta, A.M., Amato, S., Cannizzaro, N., Angiolillo, S., Arboit, M., Diamante, L., Carbognin, E., Romani, P., La Torre, F., Galimberti, E., Pflug, F., Luoni, M., Giannelli, S., Pepe, G., Capocci, L., Di Pardo, A., Vanzani, P., Zennaro, L., Broccoli, V., Leeb, M., Moro, E., Maglione, V., Martello, G. (2023) Genome-wide screening in pluripotent cells identifies Mtf1 as a suppressor of mutant huntingtin toxicity. Nature communications. 14:39623962

Badenetti, L., Manzoli, R., Rubin, M., Cozza, G., Moro, E. (2023) Monitoring Nrf2/ARE Pathway Activity with a New Zebrafish Reporter System. International Journal of Molecular Sciences. 24(7):

D'Amore, C., Moro, E., Borgo, C., Itami, K., Hirota, T., Pinna, L.A., Salvi, M. (2020) "JANUS" EFFICACY OF CX-5011: CK2 INHIBITION AND METHUOSIS INDUCTION BY INDEPENDENT MECHANISMS. Biochimica et biophysica acta. Molecular cell research. 1867(11):118807

Guglielmi, L., Bühler, A., Moro, E., Argenton, F., Poggi, L., Carl, M. (2020) Temporal control of Wnt signaling is required for habenular neuron diversity and brain asymmetry. Development (Cambridge, England). 147(6):

Costa, R., Bellesso, S., Lualdi, S., Manzoli, R., Pistorio, V., Filocamo, M., Moro, E. (2020) A transcriptional and post-transcriptional dysregulation of Dishevelled 1 and 2 underlies the Wnt signaling impairment in type I Gaucher disease experimental models. Human molecular genetics. 29:285

Costa, R., Peruzzo, R., Bachmann, M., Montà, G.D., Vicario, M., Santinon, G., Mattarei, A., Moro, E., Quintana-Cabrera, R., Scorrano, L., Zeviani, M., Vallese, F., Zoratti, M., Paradisi, C., Argenton, F., Brini, M., Calì, T., Dupont, S., Szabò, I., Leanza, L. (2019) Impaired Mitochondrial ATP Production Downregulates Wnt Signaling via ER Stress Induction. Cell Reports. 28:1949-1960.e6

Cozza, G., Moro, E., Black, M., Marin, O., Salvi, M., Venerando, A., Tagliabracci, V.S., Pinna, L.A. (2018) The Golgi "casein kinase" Fam20C is a genuine "phosvitin kinase" and phosphorylates polyserine stretches devoid of the canonical consensus. The FEBS journal. 285(24):4674-4683

Bellesso, S., Salvalaio, M., Lualdi, S., Tognon, E., Costa, R., Braghetta, P., Giraudo, C., Stramare, R., Rigon, L., Filocamo, M., Tomanin, R., Moro, E. (2018) Correction to: FGF signaling deregulation is associated with early developmental skeletal defects in animal models for mucopolysaccharidosis type II (MPSII). Human molecular genetics. 27:2407

Bellesso, S., Salvalaio, M., Lualdi, S., Tognon, E., Costa, R., Braghetta, P., Giraudo, C., Stramare, R., Rigon, L., Filocamo, M., Tomanin, R., Moro, E. (2018) FGF signaling deregulation is associated with early developmental skeletal defects in animal models for mucopolysaccharidosis type II (MPSII). Human molecular genetics. 27:2262-2275

Costa, R., Urbani, A., Salvalaio, M., Bellesso, S., Cieri, D., Zancan, I., Filocamo, M., Bonaldo, P., Szabò, I., Tomanin, R., Moro, E. (2017) Perturbations in cell signaling elicit early cardiac defects in mucopolysaccharidosis type II. Human molecular genetics. 26(9):1643-1655

Zancan, I., Bellesso, S., Costa, R., Salvalaio, M., Stroppiano, M., Hammond, C., Argenton, F., Filocamo, M., Moro, E. (2015) Glucocerebrosidase deficiency in zebrafish affects primary bone ossification through increased oxidative stress and reduced Wnt/β-catenin signaling. Human molecular genetics. 24(5):1280-94

Casari, A., Schiavone, M., Facchinello, N., Vettori, A., Meyer, D., Tiso, N., Moro, E., Argenton, F. (2014) A Smad3 transgenic reporter reveals TGF-beta control of zebrafish spinal cord development. Developmental Biology. 396(1):81-93

Kalogirou, S., Malissovas, N., Moro, E., Argenton, F., Stainier, D.Y., Beis, D. (2014) Intracardiac Flow Dynamics Regulate Atrioventricular Valve Morphogenesis. Cardiovascular research. 104(1):49-60

Kizil, C., Küchler, B., Yan, J.J., Özhan, G., Moro, E., Argenton, F., Brand, M., Weidinger, G., Antos, C.L. (2014) Simplet/Fam53b is required for Wnt signal transduction by regulating β-catenin nuclear localization. Development (Cambridge, England). 141:3529-39

Cosimelli, B., Laneri, S., Ostacolo, C., Sacchi, A., Severi, E., Porcù, E., Rampazzo, E., Moro, E., Basso, G., Viola, G. (2014) Synthesis and biological evaluation of imidazo[1,2-a]pyrimidines and imidazo[1,2-a]pyridines as new inhibitors of the Wnt/β-catenin signaling. European Journal of Medicinal Chemistry. 83C:45-56

Benato, F., Colletti, E., Skobo, T., Moro, E., Colombo, L., Argenton, F., Dalla Valle, L. (2014) A living biosensor model to dynamically trace glucocorticoid transcriptional activity during development and adult life in zebrafish. Molecular and Cellular Endocrinology. 392(1-2):60-72

Schiavone, M., Rampazzo, E., Casari, A., Battilana, G., Persano, L., Moro, E., Liu, S., Leach, S.D., Tiso, N., Argenton, F. (2014) Zebrafish reporter lines reveal in vivo signaling pathway activities involved in pancreatic cancer. Disease models & mechanisms. 7(7):883-94

Wehner, D., Cizelsky, W., Vasudevaro, M.D., Özhan, G., Haase, C., Kagermeier-Schenk, B., Röder, A., Dorsky, R.I., Moro, E., Argenton, F., Kühl, M., and Weidinger, G. (2014) Wnt/β-Catenin Signaling Defines Organizing Centers that Orchestrate Growth and Differentiation of the Regenerating Zebrafish Caudal Fin. Cell Reports. 6(3):467-481

Corallo, D., Schiavinato, A., Trapani, V., Moro, E., Argenton, F., and Bonaldo, P. (2013) Emilin3 is required for notochord sheath integrity and interacts with Scube2 to regulate notochord-derived Hedgehog signals. Development (Cambridge, England). 140(22):4594-4601

Demir, K., Kirsch, N., Beretta, C.A., Erdmann, G., Ingelfinger, D., Moro, E., Argenton, F., Carl, M., Niehrs, C., and Boutros, M. (2013) RAB8B is required for activity and caveolar endocytosis of LRP6. Cell Reports. 4(6):1224-1234

Moro, E., Vettori, A., Porazzi, P., Schiavone, M., Rampazzo, E., Casari, A., Ek, O., Facchinello, N., Astone, M., Zancan, I., Milanetto, M., Tiso, N., and Argenton, F. (2013) Generation and application of signaling pathway reporter lines in zebrafish. Molecular genetics and genomics : MGG. 288(5-6):231-242

Rampazzo, E., Persano, L., Pistollato, F., Moro, E., Frasson, C., Porazzi, P., Della Puppa, A., Bresolin, S., Battilana, G., Indraccolo, S., Te Kronnie, G., Argenton, F., Tiso, N., and Basso, G. (2013) Wnt activation promotes neuronal differentiation of Glioblastoma. Cell Death & Disease. 4:e500

Wang, X., Kopinke, D., Lin, J., McPherson, A.D., Duncan, R.N., Otsuna, H., Moro, E., Hoshijima, K., Grunwald, D.J., Argenton, F., Chien, C.B., Murtaugh, L.C., and Dorsky, R.I. (2012) Wnt signaling regulates postembryonic hypothalamic progenitor differentiation. Developmental Cell. 23(3):624-636

Hammond, C.L., and Moro, E. (2012) Using transgenic reporters to visualize bone and cartilage signaling during development in vivo. Frontiers in Experimental Endocrinology. 3:91

Dodge, M.E., Moon, J., Tuladhar, R., Lu, J., Jacob, L.S., Zhang, L.S., Shi, H., Wang, X., Moro, E., Mongera, A., Argenton, F., Karner, C.M., Carroll, T.J., Chen, C., Amatruda, J.F., and Lum, L. (2012) Diverse Chemical Scaffolds Support Direct Inhibition of the Membrane-bound O-Acyltransferase Porcupine. The Journal of biological chemistry. 287(27):23246-23254

Moro, E., Özhan, G., Mongera, A., Beis, D., Wierzbicki, C., Young, R.M., Bournele, D., Domenichini, A., Valdivia, L.E., Lum, L., Chen, C., Amatruda, J.F., Tiso, N., Weidinger, G., and Argenton, F. (2012) In vivo Wnt signaling tracing through a transgenic biosensor fish reveals novel activity domains. Developmental Biology. 366(2):327-340

Valdivia, L.E., Young, R.M., Hawkins, T.A., Stickney, H.L., Cavodeassi, F., Schwarz, Q., Pullin, L.M., Villegas, R., Moro, E., Argenton, F., Allende, M.L., and Wilson, S.W. (2011) Lef1-dependent Wnt/β-catenin signalling drives the proliferative engine that maintains tissue homeostasis during lateral line development. Development (Cambridge, England). 138(18):3931-3941

Vettori, A., Bergamin, G., Moro, E., Vazza, G., Polo, G., Tiso, N., Argenton, F., and Mostacciuolo, M.L. (2011) Developmental defects and neuromuscular alterations due to mitofusin 2 gene (MFN2) silencing in zebrafish: A new model for Charcot-Marie-Tooth type 2A neuropathy. Neuromuscular disorders : NMD. 21(1):58-67

Brennan, C., Dosch, R., Haramis, A.P., Luckenbacher, T., Martinez-Morales, J.R., Moro, E., Polok, B., Ramesh, T.M., Russell, C., Argenton, F., and Strähle, U. (2010) Report of the European Zebrafish Principal Investigator Meeting in Padua, Italy, March 18-22, 2010. Zebrafish. 7(3):305-310

Ragvin, A., Moro, E., Fredman, D., Navratilova, P., Drivenes, O., Engström, P.G., Alonso, M.E., Mustienes, E.D., Gomez Skarmeta, J.L., Tavares, M.J., Casares, F., Manzanares, M., van Heyningen, V., Molven, A., Njølstad, P.R., Argenton, F., Lenhard, B., and Becker, T.S. (2010) Long-range gene regulation links genomic type 2 diabetes and obesity risk regions to HHEX, SOX4, and IRX3. Proceedings of the National Academy of Sciences of the United States of America. 107(2):775-780

Moro, E., Tomanin, R., Friso, A., Modena, N., Tiso, N., Scarpa, M., and Argenton, F. (2010) A novel functional role of iduronate-2-sulfatase in zebrafish early development. Matrix biology : journal of the International Society for Matrix Biology. 29(1):43-50

Tiso, N., Moro, E., and Argenton, F. (2009) Zebrafish Pancreas Development. Molecular and Cellular Endocrinology. 312(1-2):24-30

Moro, E., Gnügge, L., Braghetta, P., Bortolussi, M., and Argenton, F. (2009) Analysis of beta cell proliferation dynamics in zebrafish. Developmental Biology. 332(2):299-308

Moro, E., Maran, C., Slongo, M.L., Argenton, F., Toppo, S., and Onisto, M. (2007) Zebrafish spata2 is expressed at early developmental stages. The International journal of developmental biology. 51(3):241-246

NON-ZEBRAFISH PUBLICATIONS
Wang, Z., Moro, E., Kovacs, K. Yu, R. and Melmed, S. Pituitary tumor transforming gene-null mice exhibit impaired pancreatic beta cell proliferation and diabetes. Proc Natl Acad Sci USA 2003, Mar 18;100(6):3428-32

Ferlin, A., Moro, E., Rossi, A., Dallapiccola, B., and Foresta, C. The human Y chromosome's azoospermia factor b(AZFb) region: sequence, structure, and deletion analysis in infertile men. J Med Genet 2003; 40: 18-24

Foresta, C., Bettella, A., Moro, E., Rossato, M., Merico, M., Garolla, A., and Ferlin, A. Inhibin B plasma concentrations in infertile patients with DAZ gene deletions treated with FSH. Eur J Endocrinol 2002 Jun;146(6):801-6.

Foresta, C., Bettella, A., Ferlin, A., Garolla, A., Moro, E., Baldinotti, F., Simi, P., and DallaPiccola, B. Response to local dihydrotestosterone treatment in a patient with partial androgen-insensitivity syndrome due to a novel mutation in the androgen receptor gene. American Journal of Medical Genetics, 2002, Jan 22, 107(3): 259-260.

Ferlin, A., Moro, E. (first coauthor), Rossi, A., and Foresta, C. A novel approach for the analysis of DAZ gene copy number in severely idiopathic infertile men. J Endocrinol Invest 2002 Jan;25(1):RC1-3

Marin, P., Ferlin, A., Moro, E., Rossi, A., Bartoloni, L., Rossato, M., and Foresta, C. A novel INSL3 mutation associated with human cryptorchidism. American Journal of Medical Genetics. 2001, 103 (4): 348-349.

Foresta, C., Moro, E., and Ferlin, A. Prognostic Value of Y deletion analysis. Human Reproduction 2000, 16 (8):1543-7

Foresta, C., Bettella, A., Moro, E., Merico, M., Roverato, A., and Ferlin, A. Sertoli cell function in infertile patients with and without microdeletions of the azoospermia factor on the Y chromosome. Journal of Clinical Endocrinology and Metabolism; 86 (6): 2414-2419. 2001

Marin, P., Ferlin, A., Moro, E., Garolla, A., and Foresta, C. Different insulin-like 3 (INSL3) gene mutations not associated with human cryptorchidism. Journal of Endocrinological Investigation, 2001, 24(4) RC13-5.

Maurizio, O., Romina, G., Lorenza, Z., Alessandro, N., Maurizio, M., Enrico, M., and Carlo, F. Evidence for FSH-dependent upregulation of spata2 (spermatogenesis-associated protein 2) Biochem Biophys Res Commun. Apr 27;283(1):86-92. 2001.

Foresta, C., Moro, E., and Ferlin, A. Y chromosome microdeletions and alterations of spermatogenesis. Endocrine Reviews Apr;22(2):226-39. 2001.

Ferlin, A., Moro, E., Rossi, A., and Foresta, C. CDY1 analysis in infertile patients with DAZ deletions. Journal of Endocrinological Investigation, 24: RC4-62, 2001.

Moro, E., Ferlin, A., Yen Hsiao, P., Guanciali Franchi, P., Palka, G., and Foresta, C. Male Infertility caused by a de novo partial deletion of the DAZ cluster on the Y chromosome. Journal of Clinical Endocrinology and Metabolism, 85: 4069-73, 2000.

Foresta, C., Moro, E., Rossi, A., Rossato, M., Garolla, A., and Ferlin, A. Role of the AZFa candidate genes in male infertility. Journal of Endocrinological Investigation,23, 646-51 2000.

Paracchini, S., Stuppia, L., Gatta, V., Palka, G., Moro, E., Foresta, C., Mengual, L., Oliva, R., Ballesc�, J.L., Kremer, J.A.M., van Golde, R.J.T., Tuerlings, J., Hargreave, T., Ross, A., Cooke, H., Huellen, K., Vogt, P.H., and Tyler-Smith, C. Y-chromosomal DNA haplotypes in infertile European males carrying Y-microdeletions. Journal of Endocrinological Investigation,23, 671-6. 2000.

Moro, E., Marin, P., Rossi, A., Garolla, A., and Ferlin, A. Y chromosome microdeletions in infertile men with varicocele. Molecular and Cellular Endocrinology. Mar 3; 161(1-2):67-71, 2000.

Foresta, C., Ferlin, A., and Moro, E. Deletion and expression analysis of AZFa genes on the human Y chromosome revealed a major role for DBY in male infertility. Human Molecular Genetics, May 1;143(3): 1161-9, 2000.

Foresta, C., Ferlin, A., Moro, E., and Scandellari, C. Y chromosome. Lancet 355, 234-235, 2000.

Simoni, M., Bakker, E., Eurlings, M.C.M., Matthijs, G., Moro, E., Muller, C.R., and Vogt, P.H.. Laboratory Guidelines for molecular diagnosis of for molecular diagnosis of ?chromosomal microdeletions. International Journal of Andrology 22:292-299, 1999.

Foresta, C., Moro, E., Garolla, A., Onisto, M., and Ferlin, A. Y chromosome microdeletion in cryptorchidism and idiopathic infertility. Journal of Clinical Endocrinology and Metabolism, 84: 3660-3665, 1999.

Ferlin, A., Moro, E., Onisto, M., Toscano, E., Bettella, A., and Foresta, C. Absence of testicular DAZ gene expression in idiopathic severe testiculopathies. Human Reproduction, 14:2286-2292, 1999.

Ferlin, A., Moro, E., Garolla, A., and Foresta, C. Human male infertility and Y-chromosome deletions: role of the AZF-candidate genes: DAZ, RBM, and DFFRY. Human Reproduction, 14:1710-1716, 1999.

Foresta, C., Ferlin, A., Garolla, A., Moro, E., Pistorello, M., Barbaux, S., and Rossato, M. High frequency of well-defined Y-chromosome deletions in idiopathic Sertoli cell-only syndrome. Human Reproduction, 13: 302-307, 1998.

Ferrari, S., Moro, E., Pettenazzo, A., Zacchello, F., and Scarpa, M. Exgen 500 is an efficient vector for gene delivery to lung epithelial cells in vitro and in vivo. Gene Therapy,4:1100-1106, 1997.