ZFIN Lab: Grunwald Lab

ZFIN ID: ZDB-LAB-980205-1

Grunwald Lab

PI/Director:	Grunwald, David
Contact Person:	Grunwald, David
Email:	grunwald@genetics.utah.edu
URL:	http://www.genetics.utah.edu/faculty/dgrunwald.html
Address:	Eccles Institute of Human Genetics Department of Human Genetics University of Utah Medical School 15 North 2030 East, Room 5250 Salt Lake City, UT 84112 USA
Country:	United States
Phone:	(801) 581-4442
Fax:	(801) 581-5374
Line Designation:	z

GENOMIC FEATURES ORIGINATING FROM THIS LAB

Show all 20 genomic features

STATEMENT OF RESEARCH INTERESTS

We are interested in understanding the factors that contribute to region-specific gene expression in the pre-gastrula embryo and the early events involved in the specification of differentiated tissues in the early embryo. Recently our work has focused on the role of three T-box genes that encode related transcription factors in the patterning and subdivision of the mesoderm precursor population.

LAB MEMBERS

Jurynec, Michael Graduate Student

ZEBRAFISH PUBLICATIONS OF LAB MEMBERS

Wike, C.L., Guo, Y., Tan, M., Nakamura, R., Shaw, D.K., Díaz, N., Whittaker-Tademy, A.F., Durand, N.C., Lieberman Aiden, E., Vaquerizas, J.M., Grunwald, D., Takeda, H., Cairns, B.R. (2021) Chromatin architecture transitions from zebrafish sperm through early embryogenesis. Genome research. 31(6):981-994

Arveseth, C.D., Happ, J.T., Hedeen, D.S., Zhu, J.F., Capener, J.L., Klatt Shaw, D., Deshpande, I., Liang, J., Xu, J., Stubben, S.L., Nelson, I.B., Walker, M.F., Kawakami, K., Inoue, A., Krogan, N.J., Grunwald, D.J., Hüttenhain, R., Manglik, A., Myers, B.R. (2021) Smoothened transduces Hedgehog signals via activity-dependent sequestration of PKA catalytic subunits. PLoS Biology. 19:e3001191

Teerlink, C.C., Jurynec, M.J., Hernandez, R., Stevens, J., Hughes, D.C., Brunker, C.P., Rowe, K., Grunwald, D.J., Facelli, J.C., Cannon-Albright, L.A. (2020) A role for the MEGF6 gene in predisposition to osteoporosis. Annals of human genetics. 85(2):58-72

Santoriello, C., Sporrij, A., Yang, S., Flynn, R.A., Henriques, T., Dorjsuren, B., Custo Greig, E., McCall, W., Stanhope, M.E., Fazio, M., Superdock, M., Lichtig, A., Adatto, I., Abraham, B.J., Kalocsay, M., Jurynec, M., Zhou, Y., Adelman, K., Calo, E., Zon, L.I. (2020) RNA helicase DDX21 mediates nucleotide stress responses in neural crest and melanoma cells. Nature cell biology. 22(4):372-379

Jurynec, M.J., Bai, X., Bisgrove, B.W., Jackson, H., Nechiporuk, A., Palu, R.A.S., Grunwald, H.A., Su, Y.C., Hoshijima, K., Yost, H.J., Zon, L.I., Grunwald, D.J. (2019) The Paf1 Complex and P-TEFb have reciprocal and antagonist roles in maintaining multipotent neural crest progenitors. Development (Cambridge, England). 146(24):

Hoshijima, K., Jurynec, M.J., Klatt Shaw, D., Jacobi, A.M., Behlke, M.A., Grunwald, D.J. (2019) Highly Efficient CRISPR-Cas9-Based Methods for Generating Deletion Mutations and F0 Embryos that Lack Gene Function in Zebrafish. Developmental Cell. 51(5):645-657.e4

Chagovetz, A.A., Klatt Shaw, D., Ritchie, E., Hoshijima, K., Grunwald, D.J. (2019) Interactions among Ryanodine Receptor isotypes contribute to muscle fiber type development and function. Disease models & mechanisms. 13(2):

Klatt Shaw, D., Gunther, D., Jurynec, M.J., Chagovetz, A.A., Ritchie, E., Grunwald, D.J. (2018) Intracellular Calcium Mobilization Is Required for Sonic Hedgehog Signaling. Developmental Cell. 45(4):512-525.e5

Jurynec, M.J., Sawitzke, A.D., Beals, T.C., Redd, M.J., Stevens, J., Otterud, B., Leppert, M.H., Grunwald, D.J. (2018) A hyperactivating proinflammatory RIPK2 allele associated with early-onset osteoarthritis. Human molecular genetics. 27(13):2383-2391

Morrow, Z.T., Maxwell, A.M., Hoshijima, K., Talbot, J.C., Grunwald, D.J., Amacher, S.L. (2017) tbx6l and tbx16 are redundantly required for posterior paraxial mesoderm formation during zebrafish embryogenesis. Developmental Dynamics : an official publication of the American Association of Anatomists. 246(10):759-769

Hoshijima, K., Jurynec, M.J., Grunwald, D.J. (2016) Precise Editing of the Zebrafish Genome Made Simple and Efficient. Developmental Cell. 36:654-67

Hoshijima, K., Jurynec, M.J., Grunwald, D.J. (2016) Precise genome editing by homologous recombination. Methods in cell biology. 135:121-47

Ota, S., Hisano, Y., Muraki, M., Hoshijima, K., Dahlem, T.J., Grunwald, D.J., Okada, Y., and Kawahara, A. (2013) Efficient identification of TALEN-mediated genome modifications using heteroduplex mobility assays. Genes to cells : devoted to molecular & cellular mechanisms. 18(6):450-458

Wang, X., Kopinke, D., Lin, J., McPherson, A.D., Duncan, R.N., Otsuna, H., Moro, E., Hoshijima, K., Grunwald, D.J., Argenton, F., Chien, C.B., Murtaugh, L.C., and Dorsky, R.I. (2012) Wnt signaling regulates postembryonic hypothalamic progenitor differentiation. Developmental Cell. 23(3):624-636

Dahlem, T.J., Hoshijima, K., Jurynec, M.J., Gunther, D., Starker, C.G., Locke, A.S., Weis, A.M., Voytas, D.F., and Grunwald, D.J. (2012) Simple Methods for Generating and Detecting Locus-Specific Mutations Induced with TALENs in the Zebrafish Genome. PLoS Genetics. 8(8):e1002861

Xing, L., Hoshijima, K., Grunwald, D.J., Fujimoto, E., Quist, T.S., Sneddon, J., Chien, C.B., Stevenson, T.J., and Bonkowsky, J.L. (2012) Zebrafish foxP2 Zinc Finger Nuclease Mutant Has Normal Axon Pathfinding. PLoS One. 7(8):e43968

Wythe, J.D., Jurynec, M.J., Urness, L.D., Jones, C.A., Sabeh, M.K., Werdich, A.A., Sato, M., Yost, H.J., Grunwald, D.J., MacRae, C.A., and Li, D.Y. (2011) Hadp1, a newly identified pleckstrin homology domain protein, is required for cardiac contractility in zebrafish. Disease models & mechanisms. 4(5):607-21

Demarest, B.L., Horsely, W.H., Locke, E.E., Boucher, K., Grunwald, D.J., and Trede, N.S. (2011) Trans-centromere Effects on Meiotic Recombination in the Zebrafish. Genetics. 187(1):333-336

Jurynec, M.J., and Grunwald, D.J. (2010) SHIP2, a factor associated with diet-induced obesity and insulin sensitivity, attenuates FGF signaling in vivo. Disease models & mechanisms. 3(11-12):733-742

Bai, X., Kim, J., Yang, Z., Jurynec, M.J., Akie, T.E., Lee, J., LeBlanc, J., Sessa, A., Jiang, H., DiBiase, A., Zhou, Y., Grunwald, D.J., Lin, S., Cantor, A.B., Orkin, S.H., and Zon, L.I. (2010) TIF1gamma controls erythroid cell fate by regulating transcription elongation. Cell. 142(1):133-143

Jurynec, M.J., Xia, R., Mackrill, J.J., Gunther, D., Crawford, T., Flanigan, K.M., Abramson, J.J., Howard, M.T., and Grunwald, D.J. (2008) Selenoprotein N is required for ryanodine receptor calcium release channel activity in human and zebrafish muscle. Proceedings of the National Academy of Sciences of the United States of America. 105(34):12485-12490

Vasquez, S.X., Hansen, M.S., Bahadur, A.N., Hockin, M.F., Kindlmann, G.L., Nevell, L., Wu, I.Q., Grunwald, D.J., Weinstein, D.M., Jones, G.M., Johnson, C.R., Vandeberg, J.L., Capecchi, M.R., and Keller, C. (2008) Optimization of volumetric computed tomography for skeletal analysis of model genetic organisms. The Anatomical record. 291(5):475-487

Jurynec, M.J. (2006) Mechanisms of zebrafish development and disease. Ph.D. Thesis. :162p

Lamason, R.L., Mohideen, M.A., Mest, J.R., Wong, A.C., Norton, H.L., Aros, M.C., Jurynec, M.J., Mao, X., Humphreville, V.R., Humbert, J.E., Sinha, S., Moore, J.L., Jagadeeswaran, P., Zhao, W., Ning, G., Makalowska, I., McKeigue, P.M., O'donnell, D., Kittles, R., Parra, E.J., Mangini, N.J., Grunwald, D.J., Shriver, M.D., Canfield, V.A., and Cheng, K.C. (2005) SLC24A5, a putative cation exchanger, affects pigmentation in zebrafish and humans. Science (New York, N.Y.). 310(5755):1782-1786

Hutson, L.D., Jurynec, M.J., Yeo, S.Y., Okamoto, H., and Chien, C.B. (2003) Two divergent slit1 genes in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 228(3):358-369

Goering, L.M., Hoshijima, K., Hug, B., Bisgrove, B., Kispert, A., and Grunwald, D.J. (2003) An interacting network of T-box genes directs gene expression and fate in the zebrafish mesoderm. Proceedings of the National Academy of Sciences of the United States of America. 100(16):9410-9415

Hoshijima, K., Metherall, J.E., and Grunwald, D.J. (2002) A protein disulfide isomerase expressed in the embryonic midline is required for left/right asymmetries. Genes & Development. 16(19):2518-2529

Grunwald, D.J. and Eisen, J.S. (2002) Headwaters of the zebrafish — emergence of a new model vertebrate. Nature reviews. Genetics. 3(9):717-724

Korzh, V. and Grunwald, D. (2001) Nadine Dobrovolskaia-Zavadskaia and the dawn of developmental genetics. BioEssays : news and reviews in molecular, cellular and developmental biology. 23(4):365-371

Dorsky, R.I., Snyder, A., Cretekos, C.J., Grunwald, D.J., Geisler, R., Haffter, P., Moon, R.T., and Raible, D.W. (1999) Maternal and embryonic expression of zebrafish lef1. Mechanisms of Development. 86(1-2):147-150

Cretekos, C.J. and Grunwald, D.J. (1999) alyron, an insertional mutation affecting early neural crest development in zebrafish. Developmental Biology. 210(2):322-338

Appel, B., Fritz, A., Westerfield, M., Grunwald, D.J., Eisen, J.S., and Riley, B.B. (1999) Delta-mediated specification of midline cell fates in zebrafish embryos. Current biology : CB. 9:247-256

Bisgrove, B.W., Raible, D.W., Walter, V., Eisen, J.S., and Grunwald, D.J. (1997) Expression of c-ret in the zebrafish embryo: potential roles in motoneuronal development. Journal of neurobiology. 33(6):749-768

Hug, B., Walter, V., and Grunwald, D.J. (1997) tbx6, a brachyury-related gene expressed by ventral mesendodermal precursors in the zebrafish embryo. Developmental Biology. 183(1):61-73

Liao, W., Bisgrove, B.W., Sawyer, H., Hug, B., Bell, B., Peters, K., Grunwald, D.J., and Stainier, D.Y.R. (1997) The zebrafish gene cloche acts upstream of a flk-1 homologue to regulate endothelial cell differentiation. Development (Cambridge, England). 124(2):381-389

Grunwald, D.J. (1996) A fin-de-siécle achievement: charting new waters in vertebrate biology. Science (New York, N.Y.). 274(5293):1634-1635

Riley, B.B. and Grunwald, D.J. (1996) A mutation in zebrafish affecting a localized cellular function required for normal ear development. Developmental Biology. 179(2):427-435

Weinberg, E.S., Allende, M.L., Kelly, C.S., Abdelhamid, A., Murakami, T., Andermann, P., Doerre, O.G., Grunwald, D.J., and Riggleman, B. (1996) Developmental regulation of zebrafish MyoD in wild-type, no-tail and spadetail embryos. Development (Cambridge, England). 122:271-280

Riley, B.B. and Grunwald, D.J. (1995) Efficient induction of point mutations allowing recovery of specific locus mutations in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 92:5997-6001

Helde, K.A., Wilson, E.T., Cretekos, C.J., and Grunwald, D.J. (1994) Contribution of early cells to the fate map of the zebrafish gastrula. Science (New York, N.Y.). 265:517-520

Helde, K.A. and Grunwald, D.J. (1993) The DVR-1 (Vg1) transcript of zebrafish is maternally supplied and distributed throughout the embryo. Developmental Biology. 159:418-426

Wilson, E.T., Helde, K.A., and Grunwald, D.J. (1993) Something's fishy here--Rethinking cell movements and cell fate in the zebrafish embryo. Trends in genetics : TIG. 9:348-352

Stachel, S.E., Grunwald, D.J., and Myers, P.Z. (1993) Lithium perturbation and goosecoid expression identify a dorsal specification pathway in the pregastrula zebrafish. Development (Cambridge, England). 117:1261-1274

Grunwald, D.J. and Streisinger, G. (1992) Induction of recessive lethal and specific locus mutations in the zebrafish with ethyl nitrosourea. Genetics research. 59:103-116

Grunwald, D.J., and Streisinger, G. (1992) Induction of recessive lethal mutations in zebrafish with ultraviolet light. Genetics research. 59:93-101

Helde, K. and Grunwald, D. (1992) The origin of cell diversity in zebrafish. Journal of cellular biochemistry. 16F:92

Riggleman, B. and Grunwald, D.J. (1991) Molecular cloning and analysis of three developmentally regulated genes in zebrafish N-Cam myogenin and twist. The Journal of cell biology. 115:146A

Streisinger, G., Coale, F., Taggart, C., Walker, C., and Grunwald, D.J. (1989) Clonal origin of cells in the pigmented retina of the zebrafish eye. Developmental Biology. 131(1):60-69

Grunwald, D.J., Kimmel, C.B., Westerfield, M., Walker, C., and Streisinger, G. (1988) A neural degeneration mutation that spares primary neurons in the zebrafish. Developmental Biology. 126:115-128

Grunwald, D.J., Streisinger, G., and Walker, C. (1985) Clonal origins of cells in the zebrafish eye. Journal of cellular biochemistry. (Suppl.) 9B:270

Kimmel, C.B., Grunwald, D.J., Walker, C., Westerfield, M., and Streisinger, G. (1985) Neuronal degeneration mutations that spare primary neurons in the zebrafish. Soc. Neurosci. Abstr.. 11:647

Golin, J., Grunwald, D.J., Singer, F., Walker, C., and Streisinger, G. (1982) Ethyl nitroso urea induced germline mutations in zebrafish Brachydanio-rerio. Genetics. 100:S27