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ZFIN ID: ZDB-LAB-970424-45
Laboratory of Dr. Andrew L. Miller
PI/Director: Miller, Andrew L.
Contact Person:
Email: almiller@ust.hk
URL: http://home.ust.hk/~aequorin/calcium.htm
Address: HKUST Calcium-Aequorin Imaging Laboratory Department of Biology The Hong Kong University of Science and Technology Clear Water Bay Kowloon HONG KONG
Country: Hong Kong SAR China
Phone: 852-2358-7324
Fax: 852-2358-7323
Line Designation: hkz


GENOMIC FEATURES ORIGINATING FROM THIS LAB
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STATEMENT OF RESEARCH INTERESTS
We are studying the role played by calcium ions in a wide range of fundamental biological processes. Calcium signaling is studied through the use of aequorins, a group of bioluminescent proteins. They can either be microinjected into cells or transgenically expressed without disturbing function or development. The pattern of luminescence that is emitted by an aequorin-loaded cell, tissue or organ, reveals changing patterns and levels of free calcium. We are currently studying cytokinesis in frog and fish eggs; cell cycle control in sea urchin and parchment worm eggs; injury and degeneration in squid, fish and mouse neurons, and differentiation in slime molds.


LAB MEMBERS
Webb, Sarah E. Post-Doc Cheng, Chong Nam Graduate Student Cheung, Yuk Kam Chris Graduate Student
Ma, Leung-Hang Omicron Graduate Student Tse, Pui Ying Fish Facility Staff Yim, Yun Fong Fish Facility Staff


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS
Fong, C.K., Hearn, T., Webb, S.E., Frøland Steindal, I.A., Miller, A.L., Whitmore, D. (2021) Daily rhythms in heartbeat rate are intrinsic to the zebrafish heart. Current biology : CB. 31:R239-R240
Yang, L., Webb, S.E., Jin, N., Lee, H.M., Chan, T.F., Xu, G., Chan, J.C.N., Miller, A.L., Ma, R.C.W. (2021) Investigating the role of dachshund b in the development of the pancreatic islet in zebrafish. Journal of diabetes investigation. 12(5):710-727
Jamieson, L., Waters, A., Ho, K.E., Chan, H.Y.S., Hung, J.T., Webb, S.E., Chan, C.M., Shipley, A.M., Williamson, J.G., Beer, J., Angus, C., Miller, A.L. (2020) Short-term homeostatic regulation of blood/interstitial fluid Ca2+ concentration by the scales of anadromous sea trout Salmo trutta L. during smoltification and migration. Journal of Fish Biology. 98(1):17-32
Guo, C., Webb, S.E., Chan, C.M., Miller, A.L. (2020) TPC2-mediated Ca2+ signaling is required for axon extension in caudal primary motor neurons in zebrafish embryos. Journal of Cell Science. 133(13):
Johnson, R.E., Linderman, S., Panier, T., Wee, C.L., Song, E., Herrera, K.J., Miller, A., Engert, F. (2019) Probabilistic Models of Larval Zebrafish Behavior Reveal Structure on Many Scales. Current biology : CB. 30(1):70-82.e4
Kelu, J.J., Webb, S.E., Galione, A., Miller, A.L. (2018) Characterization of ADP-ribosyl cyclase 1-like (ARC1-like) activity and NAADP signaling during slow muscle cell development in zebrafish embryos. Developmental Biology. 445(2):211-225
Tse, M.K., Hung, T.S., Chan, C.M., Wong, T., Dorothea, M., Leclerc, C., Moreau, M., Miller, A.L., Webb, S.E. (2018) Identification of Ca2+ signaling components in neural stem/progenitor cells during differentiation into neurons and glia in intact and dissociated zebrafish neurospheres.. Science China. Life sciences. 61(11):1352-1368
Xu, S., Webb, S.E., Lau, T.C.K., Cheng, S.H. (2018) Matrix metalloproteinases (MMPs) mediate leukocyte recruitment during the inflammatory phase of zebrafish heart regeneration. Scientific Reports. 8:7199
Kelu, J.J., Webb, S.E., Galione, A., Miller, A.L. (2018) TPC2-mediated Ca2+ signaling is required for the establishment of synchronized activity in developing zebrafish primary motor neurons.. Developmental Biology. 438(1):57-68
Kelu, J.J., Webb, S.E., Parrington, J., Galione, A., Miller, A.L. (2017) Ca2+ release via two-pore channel type 2 (TPC2) is required for slow muscle cell myofibrillogenesis and myotomal patterning in intact zebrafish embryos.. Developmental Biology. 425(2):109-129
Li, W.M., Chan, C.M., Miller, A.L., Lee, C.H. (2017) Dual Functional Roles of Molecular Beacon: a MicroRNA Detector and Inhibitor. The Journal of biological chemistry. 292(9):3568-3580
Webb, S.E., Miller, A.L. (2017) Ca2+ Signalling and Membrane Dynamics During Cytokinesis in Animal Cells.. Advances in experimental medicine and biology. 981:389-412
Chan, C.M., Aw, J.T., Webb, S.E., Miller, A.L. (2016) SOCE proteins, STIM1 and Orai1, are localized to the cleavage furrow during cytokinesis of the first and second cell division cycles in zebrafish embryos. Zygote (Cambridge, England). 24(6):880-889
Webb, S. (2016) LARGER, SHARPER & DEEPER. Biotechniques. 61:285-291
Barnes, M., Rensburg, G.V., Li, W.M., Mehmood, K., Mackedenski, S., Chan, C.M., King, D.T., Miller, A.L., Lee, C.H. (2015) Molecular Insights into the Coding Region Determinant-Binding Protein-RNA Interaction through Site-Directed Mutagenesis in the Heterogeneous Nuclear Ribonucleoprotein-K-homology Domains. The Journal of biological chemistry. 290(1):625-39
Kelu, J.J., Chan, H.L., Webb, S.E., Cheng, A.H., Ruas, M., Parrington, J., Galione, A., Miller, A.L. (2015) Two-Pore Channel 2 activity is required for slow muscle cell-generated Ca2+ signaling during myogenesis in intact zebrafish. The International journal of developmental biology. 59:313-325
Ma, L.H., Grove, C.L., Baker, R. (2014) Development of oculomotor circuitry independent of hox3 genes. Nature communications. 5:4221
Webb, S.E., Goulet, C., Chan, C.M., Yuen, M.Y., and Miller, A.L. (2014) Biphasic assembly of the contractile apparatus during the first two cell division cycles in zebrafish embryos. Zygote (Cambridge, England). 22:218-228
Yuen, M.Y., Webb, S.E., Chan, C.M., Thisse, B., Thisse, C., and Miller, A.L. (2013) Characterization of Ca(2+) signaling in the external yolk syncytial layer during the late blastula and early gastrula periods of zebrafish development. Biochimica et biophysica acta. Molecular cell research. 1833(7):1641-56
Chan, C.M., Miller, A.L., and Webb, S.E. (2013) Reconstitution of holo-aequorin with apoaequorin mRNA and coelenterazine in zebrafish embryos. Cold Spring Harbor protocols. 2013(5):pdb.prot072975
Webb, S.E., and Miller, A.L. (2013) Microinjecting holo-aequorin into dechorionated and intact zebrafish embryos. Cold Spring Harbor protocols. 2013(5):pdb.prot072967
Webb, S.E., Chan, C.M., and Miller, A.L. (2013) Introduction of Aequorin into Zebrafish Embryos for Recording Ca(2+) Signaling during the First 48 h of Development. Cold Spring Harbor protocols. 2013(5):383-6
Webb, S.E., and Miller, A.L. (2013) Ca(2+) signaling during activation and fertilization in the eggs of teleost fish. Cell Calcium. 53(1):24-31
Bianco, I.H., Ma, L.H., Schoppik, D., Robson, D.N., Orger, M.B., Beck, J.C., Li, J.M., Schier, A.F., Engert, F., and Baker, R. (2012) The Tangential Nucleus Controls a Gravito-inertial Vestibulo-ocular Reflex. Current biology : CB. 22(14):1285-1295
Webb, S.E., Cheung, C.C., Chan, C.M., Love, D.R., and Miller, A.L. (2012) The application of complementary luminescent and fluorescent imaging techniques to visualize nuclear and cytoplasmic Ca2+-signalling during the in vivo differentiation of slow muscle cells in zebrafish embryos under normal and dystrophic conditions. Clinical and experimental pharmacology & physiology. 39(1):78-86
Webb, S.E., Fluck, R.A., and Miller, A.L. (2011) Calcium signaling during the early development of medaka and zebrafish. Biochimie. 93(12):2112-25
Ulrich, F., Ma, L.H., Baker, R.G., and Torres-Vazquez, J. (2011) Neurovascular development in the embryonic zebrafish hindbrain. Developmental Biology. 357(1):134-51
Zhang, J., Webb, S.E., Ma, L.H., Chan, C.M., and Miller, A.L. (2011) Necessary role for intracellular Ca2+ transients in initiating the apical-basolateral thinning of enveloping layer cells during the early blastula period of zebrafish development. Development, growth & differentiation. 53(5):679-696
Webb, S.E., and Miller, A.L. (2011) Visualization of Ca2+ Signaling During Embryonic Skeletal Muscle Formation in Vertebrates. Cold Spring Harbor protocols. 3(2):a004325
Cheung, C.Y., Webb, S.E., Love, D.R., and Miller, A.L. (2011) Visualization, characterization and modulation of calcium signaling during the development of slow muscle cells in intact zebrafish embryos. The International journal of developmental biology. 55(2):153-74
Leung, C.F., Miller, A.L., Korzh, V., Chong, S.W., Sleptsova-Freidrich, I., and Webb, S.E. (2009) Visualization of stochastic Ca2+ signals in the formed somites during the early segmentation period in intact, normally developing zebrafish embryos. Development, growth & differentiation. 51(7):617-637
Mei, W., Lee, K.W., Marlow, F.L., Miller, A.L., and Mullins, M.C. (2009) hnRNP I is required to generate the Ca2+ signal that causes egg activation in zebrafish. Development (Cambridge, England). 136(17):3007-3017
Ma, L.H., Punnamoottil, B., Rinkwitz, S., and Baker, R. (2009) Mosaic hoxb4a neuronal pleiotropism in zebrafish caudal hindbrain. PLoS One. 4(6):e5944
Lam, P.Y., Webb, S.E., Leclerc, C., Moreau, M., and Miller, A.L. (2009) Inhibition of stored Ca release disrupts convergence-related cell movements in the lateral intermediate mesoderm resulting in abnormal positioning and morphology of the pronephric anlagen in intact zebrafish embryos. Development, growth & differentiation. 51(4):429-442
Ma, L.H., Webb, S.E., Chan, C.M., Zhang, J., and Miller, A.L. (2009) Establishment of a transitory dorsal-biased window of localized Ca(2+) signaling in the superficial epithelium following the mid-blastula transition in zebrafish embryos. Developmental Biology. 327(1):143-157
Cheung, Y.K. (2009) Exploring the role of Ca2+ signaling in slow muscle development using intact zebrafish embryos as an animal model. Ph.D. Thesis. :150p
Li, W.M., Webb, S.E., Chan, C.M., and Miller, A.L. (2008) Multiple roles of the furrow deepening Ca2+ transient during cytokinesis in zebrafish embryos. Developmental Biology. 316(2):228-248
Webb, S.E., Li, W.M., and Miller, A.L. (2008) Calcium signalling during the cleavage period of zebrafish development. Philosophical transactions of the Royal Society of London. Series B, Biological sciences. 363(1495):1363-1369
Webb, S.E., and Miller, A.L. (2007) Ca(2+) Signalling and early embryonic patterning during zebrafish development. Clinical and experimental pharmacology & physiology. 34(9):897-904
Ma, L.H. (2007) Characterization of calcium signals during the blastula period of zebrafish (danio rerio) embryogenesis. Ph.D. Thesis. :239p
Montaville, P., Dai, Y., Cheung, C.Y., Giller, K., Becker, S., Michalak, M., Webb, S.E., Miller, A.L., and Krebs, J. (2006) Nuclear translocation of the calcium-binding protein ALG-2 induced by the RNA-binding protein RBM22. Biochimica et biophysica acta. Molecular cell research. 1763(11):1335-1343
Webb, S.E., and Miller, A.L. (2006) Ca(2+) signaling and early embryonic patterning during the Blastula and Gastrula Periods of Zebrafish and Xenopus development. Biochimica et biophysica acta. Molecular cell research. 1763(11):1192-1208
Li, W.M., Webb, S.E., Lee, K.W., and Miller, A.L. (2006) Recruitment and SNARE-mediated fusion of vesicles in furrow membrane remodeling during cytokinesis in zebrafish embryos. Experimental cell research. 312(17):3260-3275
Lee, K.W., Webb, S.E., and Miller, A.L. (2006) Requirement for a localized, IP 3 R-generated Ca 2+ transient during the furrow positioning process in zebrafish zygotes. Zygote (Cambridge, England). 14(2):143-155
Cheung, C.Y., Webb, S.E., Meng, A., and Miller, A.L. (2006) Transient expression of apoaequorin in zebrafish embryos: extending the ability to image calcium transients during later stages of development. The International journal of developmental biology. 50(6):561-569
Cheung, Y.K. (2005) The development of alternative methods to introduce the Ca2+-sensitive bioluminescent complex, aequorin, into zebrafish embryos. Master's Thesis. :133p
Ma, O.L., Webb, S.E., and Miller, A.L. (2004) Imaging Patterns of Ca2+ Transients During the Blastula Period in Zebrafish Embryos. The Biological bulletin. 207(2):165
Miller, A.L., Leclerc, C., Moreau, M., Webb, S.E. (2004) Role of Ca2+ Signaling During Early Pronephric Development in Zebrafish and Xenopus Embryos. The Biological bulletin. 207(2):165
Cheng, J.C., Miller, A.L., and Webb, S.E. (2004) Organization and function of microfilaments during late epiboly in zebrafish embryos. Developmental dynamics : an official publication of the American Association of Anatomists. 231(2):313-323
Ma, O.L., Webb, S.E., Miller, A.L. (2004) Imaging Patterns of Ca2+ Transients During the Blastula Period in Zebrafish Embryos.. The Biological bulletin. 207:165
Miller, A.L., Leclerc, C., Moreau, M., Webb, S.E. (2004) Role of Ca2+ Signaling During Early Pronephric Development in Zebrafish and Xenopus Embryos.. The Biological bulletin. 207:165
Lee, K.W., Ho, S.M., Wong, C.H., Webb, S.E., and Miller, A.L. (2004) Characterization of mid-spindle microtubules during furrow positioning in early cleavage period zebrafish embryos. Zygote (Cambridge, England). 12(3):221-230
Lee, K.W., Webb, S.E., and Miller, A.L. (2003) Ca2+ released via IP3 receptors is required for furrow deepening during cytokinesis in zebrafish embryos. The International journal of developmental biology. 47(6):411-421
Webb, S.E. and Miller, A.L. (2003) Calcium signalling during embryonic development. Nature reviews. Molecular cell biology. 4(7):539-551
Webb, S.E. and Miller, A.L. (2003) Imaging intercellular calcium waves during late epiboly in intact zebrafish embryos. Zygote (Cambridge, England). 11(2):175-182
Lee, K.W., Webb, S.E., Ho, S.M., Wong, C.H., and Miller, A.L. (2002) A microtubule array precedes the formation of the cleavage furrow during the first two-cell division cycles in zebrafish embryos. Developmental Biology. 247(2):465-466
Cheng, J.C., Miller, A.L., and Webb, S.E. (2002) Actin-based endocytosis contributes to zebrafish epiboly. The Journal of general physiology. 120(1):46
Cheng, J.C., Miller, A.L., and Webb, S.E. (2001) The role of microfilaments in zebrafish epiboly. The Journal of general physiology. 118(1):40
Leung, C.F., Webb, S.E., and Miller, A.L. (2000) On the mechanism of ooplasmic segregation in single-cell zebrafish embryos. Development, growth & differentiation. 42(1):29-40
Webb, S.E. and Miller, A.L. (2000) Calcium signalling during zebrafish embryonic development. BioEssays : news and reviews in molecular, cellular and developmental biology. 22(2):113-123
Lee, K.W., Webb, S.E., and Miller, A.L. (1999) A wave of free cytosolic calcium traverses zebrafish eggs on activation. Developmental Biology. 214(1):168-180
Pullium, J.K., Dillehay, D.L., and Webb, S. (1999) High mortality in zebrafish (Danio rerio). Contemp. Top. Lab. Anim. Sci.. 38(3):80-83
Gilland, E., Miller, A.L., Karplus, E., Baker, R., Webb, S.E. (1999) Imaging of multicellular large-scale rhythmic calcium waves during zebrafish gastrulation. Proceedings of the National Academy of Sciences of the United States of America. 96:157-161
Leung, C.F., S.E. Webb, and A.L. Miller (1998) Calcium transients accompany ooplasmic segregation in zebrafish embryos. Development, growth & differentiation. 40:313-326
Webb, S.E., K.W. Lee, E. Karplus, and A.L. Miller (1997) Localized calcium transients accompany furrow positioning, propagation, and deepening during the early cleavage period of zebrafish embryos. Developmental Biology. 192:78-92
Lee, K.W., Baker, R., Galione, A., Gilland, E.H., Hanlon, R.T., and Miller, A.L. (1996) Ionophore-induced calcium waves activate unfertilized zebrafish (Danio rerio) eggs. The Biological bulletin. 191(2):265-267